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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pBNsp-Neo-Alpha

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 5300
Notes:
Restriction digests of the clone give the following sizes (kb): BglIII, EcoRI--3.0,1.6,0.8; SacI--2.9,2.5; EcoRI--5.4; HindIII, BamHI--2.9,2.5; Hind III--5.4. (ATCC staff) The listed cloning sites are derived from the multiple cloning site of pBluescript KS+ and are located 3' to the 3' splice site of the alpha-tubulin sequence. (personal communication) The alpha-tubulin sequences are located downstream of the PARP promoter and were derived from the immediate upstream of the ATG in the alpha-tubulin gene to the first EcoRI site located in the upstream region. (personal communication) This is an unpublished modification of BNsp-Neo-T (ATCC 37802). (ATCC staff) Linearizing with MluI before transfection is essential for efficient integration. Other restriction sites found in the polylinker region of pBluescript SK+ (for example: SpeI, NotI, SacII, EagI, BstXI, ApaII, AccI, XhoI, HindII, etc.) have not yet been tested for their presence in the insert. (personal communication) Shuttle vector for expressing foreign DNA polycistronicly from the procyclic acidic repetitive protein (PARP) promoter. Integrates at the beta- alpha tubulin tandem array. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Trypanosoma brucei, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37803
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified