Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This page is informational only.
Please contact the manufacturer for further details.
|Plasmid Type:||Insect Expression, Drosophila|
Two segments of the hsp70 gene were inserted between the EcoRI and PstI sites of pCaSpeR. The hsp70 promotor (from 87C1), extending from the XbaI site at -245 to the XmnI site at +207, was inserted upstream from the hsp70 3' region (from 87A7). This 3' region was excised from pHT4 (Schneuwly et al. Nature 325: 816-818) as a 525 bp EcoRI - PstI fragment, extending from the SalI site at the stop codon to approx. 350 bp downstream from the poly A addition site. A polylinker containing 7 unique sites (underlined) can be used to insert an open reading frame for expression. This vector is described in Thummel and Pirrotta (1992) DIS 71: 150.