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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pECV25

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 11317
Notes:
The order of the major features in this plasmid is:bla (ampR) - hph (hygR) - RSV LTR - HindIII - rabbit beta-globin intron splicing signals - NotI - agpt (G418R) - XhoI - rabbit beta-globin polyadenylation signal - SalI - oriP - SacI - EBNA-1 - ClaI. [3] Shuttle expression vector maintained episomally in human cells at a copy number of 25 - 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids. [3] The EBNA-1 gene contains an in-frame deletion of a repetitive region of 700 +/- 20 bp. This deletion increases the number of stably maintained plasmid molecules per cell. [3] Vector can be rescued from transfected cells with no detectable rearrangements. [3] Restriction digests of the clone give the following sizes (kb): HindIII/SacI--5.7 (doublet); SalI--11.5; XhoI--11.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, HeLa cells. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 77187
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified