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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pGRE5-1

Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 4000
Notes:
Plasmid to be cotransfected with plasmids encoding steroid receptors such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a reporter gene for hormone-dependent transcriptional activation. [3] Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109), sequences encoding chloramphenicol acetyltransferase, and SV40 splicing sequences. [3] Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55, 2.45; PstI--6.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, eukaryote cells. Related vectors: pGRE5-2, pGRE5CAT, pGRE5-1EBV, pGRE5-2EBV, pGRE5CATEBV. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified