Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid to be cotransfected with plasmids encoding steroid receptors such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a reporter gene for hormone-dependent transcriptional activation.  Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109), sequences encoding chloramphenicol acetyltransferase, and SV40 splicing sequences.  Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55, 2.45; PstI--6.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, eukaryote cells. Related vectors: pGRE5-2, pGRE5CAT, pGRE5-1EBV, pGRE5-2EBV, pGRE5CATEBV. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)