1

Search Vector Database | Search Addgene Plasmids

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.


Plasmid: pGW10

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Clone Method: Unknown
Size: 11594
Notes:
Created by Moore, July 1995, under contract with NCBI. Expression vector encoding cI857. (ATCC staff) The plasmid contains the following restriction sites (starting at the EcoRI site proceeding through the pBR322 sequences):HindIII--29, 5192, 9997, 10561, 10686; BamHI--375, 7601; SalI--651; PvuII--2066; PvuI--3735, 8889; EcoRI--1, 4361. (personal communication) The order of the major features in this plasmid is: EcoRI site - tetR - pMB1 ori - bla - bacteriophage lambda sequences Q, N, PR, rex, cI857, PR, Cr, O. (personal communication) The 4.3 kb BamHI fragment contains the PL and PR promoters, the cI857 gene, and 375 bp of pBR322 sequence. Distributed in aliquots of 2 ug (40 ng/ul). (ATCC staff) The insert contains the following restriction sites (approximate kb from the 5' HindIII site):BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9; EcoRI--8.0. (personal communication) Restriction digests of the clone give the following sizes (kb): HindIII--5.2, 4.8, 0.85, 0.5; BamHI--6.8, 4.3; EcoRI--6.8, 4.3; PvuII--11.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: depositor-supplied DNA, E.coli. Related vectors: pBR322, lambda. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 40167
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map