Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 2.35. (ATCC staff) Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), & SV40 (270-5171, early promoter, ori). (personal communication) A shuttle vector for expressing sequences cloned into the polylinker site under the control of the SV40 promoter. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli HB101, vertebrate cells, E.coli, E.coli DH5. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)