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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pJB61

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5557
Notes
Created by Moore, July 1995, under contract with NCBI. The insert extends from the HaeIII site at 1.4 kb on the map to the AluI site at 2.96 kb. This was constructed in a two-step process. The 5' HaeII site was linkered with EcoRI; the 3' AluI site was linkered with BamHI. The insert contains an internal BamHI site about 0.8 kb from the EcoRI site. The expression of the gene III product facilitates externalization of periplasmic protein by perturbing the permeability of the outer membrane. Medium is 1227 LB plus ampicillin. Hosts: E.coli K-38, E.coli. Related vectors: pBR322, M13. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
39162
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence