Restriction digests of the clone give the following sizes (kb): BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6. (ATCC staff) The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI. [1] Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts. The chloramphenicol resistance gene (catP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the EcoRV site. The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; ClaI--4.99. 5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39; SmaI--4.98. The order and orientations of the major features of the plasmid are: lacZ' - EcoRI/MCS/HindIII lacZ' -> - pIP404 ori - <- rep - <- catP - pMB1 ori. [2] Growth: LB plus chloramphenicol (10 ug/ml) (ATCC med num 1675) 37C Deposited by: Rood J.I. Hosts: E.coli, Clostridium perfringens. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)