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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pLTRpoly

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 4273
Notes:
Expression shuttle vector with promoters for in vitro RNA synthesis. (personal communication) Consists of the following sequences: nt 1-51 from pGEM7Zf(+) (reverse of 28-78), 52-70 from pUC19 (reverse of 428-446), 71-1090 from pSV2neo (reverse of 3050-4069), 1091-3474 from pSP72 (88-2462 and 1-9), (personal communication) and 3475-4273 from pMP-1 (with 7505-8297 from MoMuLV). (personal communication) The order of the major features in this plasmid is: HindIII site of MCS (nt = 1) - SV40 splice acceptor - SV40 late polyadenylation signal - T7 promoter - bla - SP6 promoter - XhoI site - MoMuLV LTR. (personal communication) Note that the T7 and SP6 promoters are not immediately adjacent to the cloning sites. (personal communication) When cotransfected with pSV2-neo (ATCC 37149) into NIH 3T3 cells, 70-80% of G418-resistant colonies expressed high levels of appropriately sized mRNA. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; PstI--4.3; XhoI--3.6; 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: mouse, E.coli HB101, E.coli DH5alpha, vertebrate cells, E.coli. Related vectors: pGEM-7Zf+, pUC19, pSV2neo, pSP72, pMP-1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 77109
GenBank: X60280
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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