Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Expression shuttle vector with promoters for in vitro RNA synthesis. (personal communication) Consists of the following sequences: nt 1-51 from pGEM7Zf(+) (reverse of 28-78), 52-70 from pUC19 (reverse of 428-446), 71-1090 from pSV2neo (reverse of 3050-4069), 1091-3474 from pSP72 (88-2462 and 1-9), (personal communication) and 3475-4273 from pMP-1 (with 7505-8297 from MoMuLV). (personal communication) The order of the major features in this plasmid is: HindIII site of MCS (nt = 1) - SV40 splice acceptor - SV40 late polyadenylation signal - T7 promoter - bla - SP6 promoter - XhoI site - MoMuLV LTR. (personal communication) Note that the T7 and SP6 promoters are not immediately adjacent to the cloning sites. (personal communication) When cotransfected with pSV2-neo (ATCC 37149) into NIH 3T3 cells, 70-80% of G418-resistant colonies expressed high levels of appropriately sized mRNA. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; PstI--4.3; XhoI--3.6; 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: mouse, E.coli HB101, E.coli DH5alpha, vertebrate cells, E.coli. Related vectors: pGEM-7Zf+, pUC19, pSV2neo, pSP72, pMP-1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)