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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Restriction digests of the clone give the following sizes (kb): PvuI--3.4, 1.8; EcoRI--2.6, 2.6; BglII--5.2; ClaI--4.1, 1.1. (ATCC staff) Expression vector containing two multiple cloning sites: the first being within the lacZ' gene, and the second being flanked by an RSV enhancer-MMTV LTR promoter at one end and splicing and polyadenylation signals at the other.  The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation.  The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2640; ClaI--425, 1560; DrdI--1398, 3152, 5021; BglII--1605; SphI--2476; NsiI--2478; PvuI--2522, 4310; HindIII--2691; BsaI--4010. (personal communication) The order of the major features in this plasmid is: NotI/EcoRI - 5' RSV enhancer - MMTV LTR 3' - ClaI/MCSII'/BglII - splicing - polyadenylation - NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB1 ori - ampR.  Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM105, E.coli JM109, vertebrate cells. Related vectors: pMAMneo, pS1, pK. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)