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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pM

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 5166
Notes:
Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Restriction digests of the clone give the following sizes (kb): PvuI--3.4, 1.8; EcoRI--2.6, 2.6; BglII--5.2; ClaI--4.1, 1.1. (ATCC staff) Expression vector containing two multiple cloning sites: the first being within the lacZ' gene, and the second being flanked by an RSV enhancer-MMTV LTR promoter at one end and splicing and polyadenylation signals at the other. [1] The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation. [1] The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2640; ClaI--425, 1560; DrdI--1398, 3152, 5021; BglII--1605; SphI--2476; NsiI--2478; PvuI--2522, 4310; HindIII--2691; BsaI--4010. (personal communication) The order of the major features in this plasmid is: NotI/EcoRI - 5' RSV enhancer - MMTV LTR 3' - ClaI/MCSII'/BglII - splicing - polyadenylation - NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB1 ori - ampR. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM105, E.coli JM109, vertebrate cells. Related vectors: pMAMneo, pS1, pK. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 77383
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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