Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMSEx-1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5600
- Notes
- Kanamycin resistance should be checked at regular intervals. This is best done during amplification steps using LB medium supplemented with 50 ug/ml ampicillin and 12.5 ug/ml kanamycin. (personal communication) The SV40 promoter has a 3 bp deletion at the BglI site within the origin of replication to prevent autonomous replication in the presence of SV40 large T-antigen. The MSV LTR provides a polyadenylation signal as well as an enhancer for the P1/thymidine kinase promoter regulating the Tn5 kanR/G418R transcription unit. One of 3 shuttle expression vectors (pMSEx-1-3, ATCC 37835-37837) containing a synthetic oligonucleotide providing a translation initiation signal, followed by a StuI site in 3 different positions, followed by a stop signal in all reading frames. The sequence of the oligonucleotide is: AGCTTGGTAC CATGGACTCG AGGCCTAGCT AGCTAGGAGCT. A EcoRI/HindIII digest gives fragments of 2.63, 1.68, 0.97 and 0.32 kb. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.4, 1.65, 1.05, 0.5; EcoRI--4.1, 1.5; BglII--5.6; BglI--4.4, 1.1; uncut. (ATCC staff) The order of the major features in this plasmid is:SV40 promoter - oligonucleotide/StuI/oligonucleotide - MLV-LTR - P1 promoter - TK promoter - kanR/G418R - TK polyadenylation signal - ampR. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37835
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified