Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pSP1

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 6700
Notes:
Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed. The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: G.F.Cottarel Hosts: Schizosaccharomyces pombe, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 77497
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified