Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed. The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1.  Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: G.F.Cottarel Hosts: Schizosaccharomyces pombe, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)