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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pT181

Information

Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4437
Notes
pT181 occurs naturally in staphylococcus aureus and encodes inducible resistance to tetracycline. it contains four open reading frames encoding four putative polypeptides a, 37.5 kda; b, 35 kda; c, 23 kda, and d, 18 kda [3]. polypeptide a is the repc protein [2] (bases 3563-557). polypeptides b and d (bases 2595-3482 and 2180-2638, respectively) are involved in tetracycline resistance [3]. there is no known function for polypeptide c and deletion of a large portion of it causes no noticeable changes [3]. Replication, originating in a 168 bp span, proceeds unidirectionally and clockwise (anticlockwise as the map is conventionally drawn) [2]. Hosts: Staphylococcus aureus, Staphylococcus. Related vectors: Staphylococcus, pC221, pE194. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence