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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
Plasmid: pUAST
This vector is NOT available from Addgene.
This page is informational only.
Please contact the manufacturer for further details.
| Analyze: | Sequence |
| Plasmid Type: | Insect Expression, Drosophila |
| Cloning Method: | Unknown |
| Size: | 8904 |
| Bacterial Resistance: | Ampicillin |
| Notes: | Brand and Perimon (1993) Development 118 401-415
pUAST consists of five tandemly arrayed optimized GAL4 binding sites followed by the hsp70 TATA box and transcriptional start , a polylinker containing unique restriction sites for EcoRI, BglII, NotI, Xho, KpnI and XbaI and the SV40 small T intron and polyadenylation site. These features are included in a P-element vector (pCaSpeR3)containing the P element ends (P3' and P5') and the white gene which acts as a marker for successful incorporation into the Drosophila genome.
More information: Brand and Perimon (1993) Development 118 401-415
P element-based vector for Gal4-regulated expression of genes in Drosophila.
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| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Unspecified |