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5WCJ (METTL13)
(Plasmid #101270)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101270 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL13
  • Alt name
    5WCJ
  • Alt name
    gi|55956896
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    690
  • Entrez Gene
    METTL13 (a.k.a. feat, CGI-01, FLJ10310, KIAA0859, 5630401D24Rik)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5WCJ (METTL13) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101270 ; http://n2t.net/addgene:101270 ; RRID:Addgene_101270)