pMOD_A0101
(Plasmid
#101747)
-
PurposeAtcas9
-
Depositing Lab
-
Depositing OrganizationClemson University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepCAMBIA
-
Backbone manufacturerUBMTA
- Backbone size w/o insert (bp) 10658
- Total vector size (bp) 10658
-
Vector typePlant Expression, CRISPR
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameT-DNA
-
SpeciesH. sapiens (human); Cotton and Medicago
-
Insert Size (bp)10658
- Promoter 35S
-
Tags
/ Fusion Proteins
- unknown (C terminal on backbone)
- CAS9 (C terminal on backbone)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer UNKNOWN
- 3′ sequencing primer UNKNOWN
- (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameCAS9
-
SpeciesSynthetic
- Promoter 35S
-
Tag
/ Fusion Protein
- CAS9 (C terminal on backbone)
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer UNKNOWN
- 3′ sequencing primer UNKNOWN
- (Common Sequencing Primers)
Resource Information
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMOD_A0101 was a gift from Christopher Saski (Addgene plasmid # 101747) -
For your References section:
A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548