A multi-purpose toolkit to enable advanced genome engineering in plants.
Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. PubMed Article
Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90997 | pMOD_A0000 | Module A, Promoter: none, Gene: none, Terminator: none |
| 90998 | pMOD_A0101 | Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP |
| 91000 | pMOD_A0103 | Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP |
| 91001 | pMOD_A0108 | Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP |
| 91002 | pMOD_A0201 | Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP |
| 91003 | pMOD_A0202 | Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP |
| 91004 | pMOD_A0208 | Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP |
| 91005 | pMOD_A0301 | Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP |
| 91006 | pMOD_A0302 | Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP |
| 91007 | pMOD_A0308 | Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP |
| 91009 | pMOD_A0402 | Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP |
| 91010 | pMOD_A0408 | Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP |
| 91011 | pMOD_A0501 | Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSP |
| 91012 | pMOD_A0502 | Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP |
| 91013 | pMOD_A0503 | Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP |
| 91014 | pMOD_A0508 | Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP |
| 91016 | pMOD_A0601 | Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP |
| 91018 | pMOD_A0608 | Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP |
| 91019 | pMOD_A0701 | Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP |
| 91021 | pMOD_A0708 | Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP |
| 91022 | pMOD_A0801 | Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP |
| 91023 | pMOD_A0802 | Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP |
| 91024 | pMOD_A0808 | Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP |
| 91025 | pMOD_A0901 | Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP |
| 91026 | pMOD_A0902 | Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP |
| 91027 | pMOD_A0908 | Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP |
| 91029 | pMOD_A1002 | Module A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2) |
| 91030 | pMOD_A1010 | Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2) |
| 91031 | pMOD_A1110 | Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP |
| 91032 | pMOD_A1111 | Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP |
| 91033 | pMOD_A1210 | Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP |
| 91034 | pMOD_A1310 | Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP |
| 91035 | pMOD_A1410 | Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP |
| 91036 | pMOD_A1510 | Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP |
| 91037 | pMOD_A1511 | Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP |
| 91038 | pMOD_A1610 | Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP |
| 91039 | pMOD_A1710 | Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP |
| 91040 | pMOD_A1810 | Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP |
| 91041 | pMOD_A1910 | Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP |
| 91042 | pMOD_A3001 | Module A, Promoter: 35S, Gene: GFP, Terminator: HSP |
| 91043 | pMOD_A3003 | Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP |
| 91044 | pMOD_A3010 | Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP |
| 91045 | pMOD_A3012 | Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP |
| 91046 | pMOD_A3013 | Module A, Promoter: PvUbi2, Gene: GFP, Terminator: HSP |
| 91047 | pMOD_A3014 | Module A, Promoter: Nos, Gene: GFP, Terminator: HSP |
| 91048 | pMOD_A3101 | Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2) |
| 91049 | pMOD_A3301 | Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2) |
| 91050 | pMOD_A3501 | Module A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSP |
| 91051 | pMOD_A3601 | Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP |
| 91052 | pMOD_A3701 | Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP |
| 91053 | pMOD_A3801 | Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VP64, Terminator: HSP |
| 91054 | pMOD_A3910 | Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP |
| 91055 | pMOD_A4010 | Module A, Promoter: ZmUbi, Gene: dTaCas9-VP64, Terminator: HSP |
| 91056 | pMOD_A4110 | Module A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSP |
| 91057 | pMOD_A4210 | Module A, Promoter: Zmubi, Gene: Csy4-P2A-dTaCas9-VP64, Terminator: HSP |
| 91058 | pMOD_B0000 | Module B, Promoter: none, Gene: none, Terminator: none |
| 91059 | pMOD_B2000 | Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP |
| 91060 | pMOD_B2101 | Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S |
| 91061 | pMOD_B2103 | Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S |
| 91062 | pMOD_B2103b | Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S |
| 91064 | pMOD_B2112 | Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S |
| 91065 | pMOD_B2121 | Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S |
| 91066 | pMOD_B2203 | Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C |
| 91067 | pMOD_B2301 | Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S |
| 91068 | pMOD_B2303 | Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S |
| 91069 | pMOD_B2309 | Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S |
| 91070 | pMOD_B2312 | Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S |
| 91071 | pMOD_B2403 | Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S |
| 91072 | pMOD_B2515 | Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91073 | pMOD_B2515b | Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III |
| 91074 | pMOD_B2517 | Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91075 | pMOD_B2518 | Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91076 | pMOD_B2519 | Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91077 | pMOD_B2520 | Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91078 | pMOD_B2615 | Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III |
| 91079 | pMOD_B3200 | Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP |
| 91080 | pMOD_B3400 | Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP |
| 91081 | pMOD_C0000 | Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none |
| 91082 | pMOD_C2200 | Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S |
| 91083 | pMOD_C2515 | Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91084 | pMOD_C2516 | Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91085 | pMOD_C2516b | Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91086 | pMOD_C2517 | Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91087 | pMOD_C2518 | Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91088 | pMOD_C2519 | Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91089 | pMOD_C2520 | Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91090 | pMOD_C2616 | Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III |
| 91091 | pMOD_C2806 | Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91092 | pMOD_C2813 | Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91093 | pMOD_C2906 | Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91094 | pMOD_C3001 | Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91095 | pMOD_C3003 | Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91096 | pMOD_C3006 | Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91098 | pMOD_C3010 | Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91099 | pMOD_C3012 | Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91100 | pMOD_C3013 | Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9 |
| 91101 | pMOD_C3106 | Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP |
| 91102 | pMOD_C3306 | Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP |
| 91103 | pMOD_C4300 | Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none |
| 91105 | pTRANS_201 | Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV |
| 91106 | pTRANS_202 | Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV |
| 91108 | pTRANS_210 | Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none |
| 91109 | pTRANS_210d | Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none |
| 91110 | pTRANS_211 | Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV |
| 91111 | pTRANS_212 | Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV |
| 91112 | pTRANS_213 | Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV |
| 91113 | pTRANS_220 | Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none |
| 91114 | pTRANS_220d | Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none |
| 91115 | pTRANS_221 | Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV |
| 91116 | pTRANS_222 | Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV |
| 91117 | pTRANS_223 | Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV |
| 91120 | pTRANS_231 | Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV |
| 91121 | pTRANS_232 | Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV |
| 91122 | pTRANS_233 | Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV |
| 91125 | pTRANS_253 | Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV |
| 91126 | pTRANS_260d | Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none |
| 91127 | pTRANS_263 | Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV |
| 91128 | pDIRECT_21A | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II |
| 91129 | pDIRECT_21B | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II |
| 91130 | pDIRECT_21C | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II |
| 91131 | pDIRECT_21D | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II |
| 91132 | pDIRECT_21J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II |
| 91133 | pDIRECT_22A | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II |
| 91135 | pDIRECT_22C | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II |
| 91136 | pDIRECT_22D | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II |
| 91137 | pDIRECT_22J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:npt II |
| 91138 | pDIRECT_23A | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar |
| 91140 | pDIRECT_23C | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar |
| 91141 | pDIRECT_23D | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar |
| 91142 | pDIRECT_23J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:bar |
| 91143 | pDIRECT_25F | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II |
| 91144 | pDIRECT_25G | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II |
| 91145 | pDIRECT_25H | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II |
| 91146 | pDIRECT_25I | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II |
| 91147 | pDIRECT_25K | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt II |
| 91149 | pDIRECT_26G | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar |
| 91150 | pDIRECT_26H | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar |
| 91152 | pDIRECT_26K | Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:bar |
| 91153 | pDIRECT_37J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II |
| 91154 | pDIRECT_38J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt II |
| 91155 | pDIRECT_39J | Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:bar |
| 91156 | pAtCas9_1 | Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II |
| 91158 | pAtCas9_3 | Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar |
| 91159 | pAtCas9-D10A_1 | Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II |
| 91160 | pAtCas9-D10A_2 | Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II |
| 91161 | pAtCas9-D10A_3 | Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar |
| 91162 | pAtCas9-H840A_1 | Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II |
| 91163 | pAtCas9-H840A_2 | Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II |
| 91165 | pAtCas9-dead_1 | Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II |
| 91166 | pAtCas9-dead_2 | Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II |
| 91167 | pAtCas9-dead_3 | Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar |
| 91168 | pTaCas9_5 | Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II |
| 91169 | pTaCas9_6 | Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar |
| 91170 | pTaCas9-D10A_5 | Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II |
| 91171 | pTaCas9-D10A_6 | Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar |
| 91172 | pTaCas9-H840A_5 | Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II |
| 91173 | pTaCas9-H840A_6 | Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar |
| 91174 | pTaCas9-dead_5 | Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II |
| 91175 | pTaCas9-dead_6 | Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar |
| 91176 | pTC102 | a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots |
| 91178 | pTC130 | BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000) |
| 91179 | pSC1 | T-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650A |
| 91180 | pSC2 | T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter) |
| 91181 | pSC3 | T-DNA vector for targeted deletion of 58kb region in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter) |
| 91182 | pSC4 | T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter) |
| 91183 | pJG363 | BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor) |
| 91185 | pJG367 | BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor) |
| 91187 | pJG370 | BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor) |
| 91188 | pJG380 | BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor) |
| 91189 | pJG382 | BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor) |
| 91194 | pJG484 | WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor) |
| 91195 | pJG486 | WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor) |
| 91196 | pJG488 | WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor) |
| 91197 | pJG493 | WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor) |
| 91198 | pTRANS_100 | Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none |
| 91199 | pTRANS_101 | Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV |
| 91200 | pTRANS_102 | Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV |
| 91202 | pTRANS_110 | Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none |
| 91203 | pTRANS_120 | Transformation, Type: non-T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none |
| 91204 | pTRANS_130 | Transformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none |
| 91205 | pTRANS_150 | Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none |
| 91206 | pTRANS_160 | Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none |
| 91207 | pDIRECT_10A | Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none |
| 91209 | pDIRECT_10E | Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none |
| 91210 | pTC303 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters) |
| 91211 | pTC363 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters) |
| 91212 | pAH750 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds) |
| 91213 | pTC364 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing) |
| 91214 | pTC365 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing) |
| 91215 | pTC366 | protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing) |
| 91216 | pTC362 | protoplast vector for targeted deletion of 6 genes in tomato |
| 91218 | pTC441 | protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array) |
| 91219 | pTC437 | protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array) |
| 91220 | pTC391 | protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression) |
| 91221 | pTC393 | protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression) |
| 91222 | pTC436 | protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression) |
| 91223 | pTC392 | protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression) |
| 91224 | pTC394 | protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression) |
| 91225 | pTC440 | protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression) |