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GST-BHMT-I/G
(Plasmid #104442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104442 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 104442-D50 50 μg of DNA in Tris buffer $495
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DNA 104442-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4754
  • Total vector size (bp) 7832
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BHMT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3213
  • Mutation
    N43A in GST (in a potential KFERQ-like sequence). BHMT does not contain the first 8 or last 9 amino acids.
  • Entrez Gene
    BHMT (a.k.a. BHMT1, HEL-S-61p)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • GFP-myc GFP is from Aequorea victoria (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nru1 (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer SP6 5'-TGATGAGTTTGGACAAACCAC-3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP is from Aequorea victoria

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-BHMT-I/G was a gift from Carol Mercer (Addgene plasmid # 104442 ; http://n2t.net/addgene:104442 ; RRID:Addgene_104442)

  • For your References section:

    Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization. Mercer CA, Kaliappan A, Dennis PB. Autophagy. 2008 Feb;4(2):185-94. Epub 2007 Nov 7. 5275 [pii] PubMed 18059170
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