pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
(Plasmid
#105000)
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PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
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Depositing Lab
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Depositing OrganizationFriedrich Miescher Institute for Biomedical Research
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 105000-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 105000-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSF4
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla luciferase
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SpeciesRenilla reniformis
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Insert Size (bp)930
- Promoter TetCMV
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Tags
/ Fusion Proteins
- 24xGCN4 repeats (SunTag) (N terminal on insert)
- PreScission protease site-Rh1-RILPL2 (C terminal on insert)
- 24xPP7v3 stem loops in 3'UTR
- xrRNA1-xrRNA2 in 3'UTR
- 24xMS2v5 stem loops in 3'UTR
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AATACGACTCACTATAGGC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note plasmid contains 24xGCN4 repeats and not 20xGCN4 repeats as indicated in the name.
DNA (Catalog # 105000-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 105000-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA was a gift from Jeffrey Chao (Addgene plasmid # 105000 ; http://n2t.net/addgene:105000 ; RRID:Addgene_105000) -
For your References section:
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824
