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Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum.
Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF
Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104982phage UbiC SNAP-KDELLentiviral vector expressing SNAP-KDEL for ER labeling
104984pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyAExpresses ST-Renilla reporter in mammalian cells
104998phage UbiC scAB-GFPLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling
104999phage UbiC NLS HA stdMCP stdHaloLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
105000pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyAExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
107230pUC57-PP7v3Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells