Skip to main content
Addgene
Open Menu

pAd-Track Flag-SIRT1-DeltaExon2
(Plasmid #105685)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105685 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pADTrack-CMV
  • Backbone size w/o insert (bp) 9220
  • Total vector size (bp) 11317
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NP_001153061.1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2097
  • Mutation
    Synonymous base changes not affecting the protein sequence
  • Entrez Gene
    Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAd-Track Flag-SIRT1 (Plasmid #8438) was obtained from Addgene and was modified to generate pAd-Track Flag-SIRT1-DeltaExon2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track Flag-SIRT1-DeltaExon2 was a gift from Ullas Kolthur-Seetharam (Addgene plasmid # 105685)

  • For your References section:

    Identification of a Tissue-Restricted Isoform of SIRT1 Defines a Regulatory Domain that Encodes Specificity. Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, Kolthur-Seetharam U. Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 10.1016/j.celrep.2017.03.012. 10.1016/j.celrep.2017.03.012 PubMed 28355560
Related items: