Identification of a Tissue-Restricted Isoform of SIRT1 Defines a Regulatory Domain that Encodes Specificity.
Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, Kolthur-Seetharam U
Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 10.1016/j.celrep.2017.03.012. PubMed Article
Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, Kolthur-Seetharam U
Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 10.1016/j.celrep.2017.03.012. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105670 | pcDNA 3.1 SIRT1-FL | Mammalian expression construct of mouse SIRT1 full length isoform |
| 105671 | pcDNA 3.1 SIRT1-deltaExon2 | Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2) |
| 105680 | pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3 | Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3 |
| 105681 | pGEX-4T-3 SIRT1-NTerm Exon1-Exon3 | Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2) |