PurposeExpresses Ag43 under the control of an L-arabinose transcriptional regulator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107743||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufactureriGEM BioBricks
- Backbone size w/o insert (bp) 4549
- Total vector size (bp) 7833
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)3284
Mutationremoved all pstI cut sites with silent mutations, added RBS and terminator
Entrez Geneflu (a.k.a. b2000, ECK1993, agn, agn43, yeeQ, yzzX)
- Promoter pBAD
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer attaccgcctttgagtgagc
- 3′ sequencing primer GCGGTCGGTCGATAAAAAAATCG (Common Sequencing Primers)
Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD-Ag43 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 107743 ; http://n2t.net/addgene:107743 ; RRID:Addgene_107743)
For your References section:Biofilm Lithography enables high-resolution cell patterning via optogenetic adhesin expression. Jin X, Riedel-Kruse IH. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3698-3703. doi: 10.1073/pnas.1720676115. Epub 2018 Mar 19. 10.1073/pnas.1720676115 PubMed 29555779