PurposeExpresses Ag43 under the control of a blue-light transcriptional regulator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107742||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAndreas Moeglich Lab
- Backbone size w/o insert (bp) 7293
- Total vector size (bp) 10440
Modifications to backbonereplaced KanR with specR
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)3147
Mutationremoved all pstI cut sites with silent mutations, added RBS
Entrez Geneflu (a.k.a. b2000, ECK1993, agn, agn43, yeeQ, yzzX)
- Promoter pLambda
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer ctctggcggtgataatggttgc
- 3′ sequencing primer GAGCCCCCGATTTAGAGC (Common Sequencing Primers)
Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDawn-Ag43 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 107742 ; http://n2t.net/addgene:107742 ; RRID:Addgene_107742)
For your References section:Biofilm Lithography enables high-resolution cell patterning via optogenetic adhesin expression. Jin X, Riedel-Kruse IH. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3698-3703. doi: 10.1073/pnas.1720676115. Epub 2018 Mar 19. 10.1073/pnas.1720676115 PubMed 29555779