|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43795||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 3′ sequencing primer T7term (Common Sequencing Primers)
NOTE: there is a second, non-annotated EcoRI site present in this plasmid. The function of the plasmid will not be impaired; however,
you cannot use the EcoRI site in the MCS for cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDusk was a gift from Andreas Moeglich (Addgene plasmid # 43795 ; http://n2t.net/addgene:43795 ; RRID:Addgene_43795)
For your References section:From dusk till dawn: one-plasmid systems for light-regulated gene expression. Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A. J Mol Biol. 2012 Mar 2;416(4):534-42. doi: 10.1016/j.jmb.2012.01.001. Epub 2012 Jan 8. 10.1016/j.jmb.2012.01.001 PubMed 22245580