Purposeheterologous expression of MVK of M. mazei in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108947||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameMevalonate kinase (MVK)
/ Fusion Protein
- His6-Tag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
codon-optimized synthetic gene for expression in E. coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-28a(+)-MVK mazei was a gift from Frank Schulz (Addgene plasmid # 108947 ; http://n2t.net/addgene:108947 ; RRID:Addgene_108947)
For your References section:An in vitro Biosynthesis of Sesquiterpenes starting from Acetic Acid. Schulz F, Dirkmann M, Nowack J. Chembiochem. 2018 Aug 7. doi: 10.1002/cbic.201800128. 10.1002/cbic.201800128 PubMed 30085399