|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110224||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. pombe (fission yeast)
MutationSee depositor comments below.
Entrez Geneura4 (a.k.a. SPCC330.05c)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
Addgene NGS results detected a P127S variant in Ura4. This variant does not affect plasmid function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAW12 was a gift from Tony Carr (Addgene plasmid # 110224 ; http://n2t.net/addgene:110224 ; RRID:Addgene_110224)
For your References section:Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe. Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.. Gene. 2008 Jan 15;407(1-2):63-74 10.1016/j.gene.2007.09.024 PubMed 18054176