Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe.
Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
Gene. 2008 Jan 15;407(1-2):63-74 PubMed Article
Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
Gene. 2008 Jan 15;407(1-2):63-74 PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110220 | pAW1 | integration of loxP-ura4+-loxM3 cassette |
| 110221 | pAW5 | Cre-expression plasmid |
| 110222 | pAW8 | Cre-expression plasmid for cassette exchange |
| 110223 | pAW8_Xho | Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8 |
| 110224 | pAW12 | integration of ura4+-loxM3 cassette |
| 110225 | pAW32 | integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter |
| 110226 | pAW33 | integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter |
| 110227 | pAW34 | integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter |
| 110228 | pAW41 | integration of loxP site a distance upstream of gene start site |
| 110229 | pAW8_yEGFP | Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein |
| 110231 | pAW8_yECitrine | Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein |
| 110232 | pAW8_13Myc | Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope |
| 110233 | pAW8_3HA | Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope |
| 110234 | pAW8_1XFLAG | Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope |
| 110235 | pAW8_6XFLAG | Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope |
| 110236 | pAW8_6XHis | Cre/Lox C-terminal tagging construct encoding six histidine (His) residues |
| 110237 | pAW8_GST | Cre/Lox C-terminal tagging construct encoding glutathione S-transferase |
| 110238 | pAW8_mCherry | Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry |
| 110239 | pAW8_tdTomato | Cre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato |
| 110240 | pAW8_TAP | Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein |