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Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe.
Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
Gene. 2008 Jan 15;407(1-2):63-74
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110220pAW1integration of loxP-ura4+-loxM3 cassette
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110221pAW5Cre-expression plasmid
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110222pAW8Cre-expression plasmid for cassette exchange
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110223pAW8_XhoCre-expression plasmid for cassette exchange but MCS modified compared to pAW8
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110224pAW12integration of ura4+-loxM3 cassette
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110225pAW32integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter
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110226pAW33integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter
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110227pAW34integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter
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110228pAW41integration of loxP site a distance upstream of gene start site
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110229pAW8_yEGFPCre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein
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110231pAW8_yECitrineCre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein
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110232pAW8_13MycCre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope
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110233pAW8_3HACre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope
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110234pAW8_1XFLAGCre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope
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110235pAW8_6XFLAGCre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope
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110236pAW8_6XHisCre/Lox C-terminal tagging construct encoding six histidine (His) residues
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110237pAW8_GSTCre/Lox C-terminal tagging construct encoding glutathione S-transferase
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110238pAW8_mCherryCre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry
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110239pAW8_tdTomatoCre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato
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110240pAW8_TAPCre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein
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