|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113689||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAgilent Technologies
- Total vector size (bp) 7148
Vector typeAAV, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
/ Fusion Proteins
- KASH (C terminal on insert)
- HAx2 (C terminal on insert)
- Flag (C terminal on insert)
- NLS (N terminal on insert)
- NLS (C terminal on insert)
A portion of this plasmid was derived from a plasmid made bypX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA from Feng Zhang plasmid #61591.
Terms and Licenses
P2A-HAFLAGHA-KASH was cloned from a custom gBlock.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA was a gift from Jonathan Ploski (Addgene plasmid # 113689 ; http://n2t.net/addgene:113689 ; RRID:Addgene_113689)
For your References section:The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Kumar N, Stanford W, Aradhana CS, Abraham ND, Dao TJ, Thaseen S, Sairavi A, Gonzalez CU and Ploski JE.. Front. Mol. Neurosci. (2018) 13 10.3389/fnmol.2018.00413