pLenti6.3/V5-hM3D(Gq)-mCherry
(Plasmid #113975)

• Purpose: For packaging the hM3D(Gq)-mCherry construct in to lentivirus for injection or stable cell line creation.
• Depositing Lab: Bryan Roth
• Depositing Organization: University of North Carolina at Chapel Hill
  Located in the United States of America
• Publication: pLenti6.3/V5-hM3D(Gq)-mCherry (unpublished)

Backbone

• Vector backbone: pLenti6.3/V5
• Backbone size w/o insert (bp): 7500
• Total vector size (bp): 10000
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hM3DG(Gq)-mCherry
• Alt name: Gq DREADD
• Species: chimeric sequence
• Insert Size (bp): 2500
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.