|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11415||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namedisulfide oxidoreductase
Insert Size (bp)255
GenBank IDAAB98293 NP_247280 MJ0307
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pET21a.MJ0307 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a.MJ0307 was a gift from Sung-Hou Kim (Addgene plasmid # 11415)
For your References section:Solution nuclear magnetic resonance structure of a protein disulfide oxidoreductase from Methanococcus jannaschii. Cave JW, Cho HS, Batchelder AM, Yokota H, Kim R, Wemmer DE. Protein Sci. 2001 Feb . 10(2):384-96. 10.1110/ps.35101 PubMed 11266624