Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22468||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerPMID 9558351)
- Backbone size w/o insert (bp) 5279
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namecytochrome c
SpeciesH. sapiens (human)
Insert Size (bp)321
Entrez GeneCYCS (a.k.a. CYC, HCS, THC4)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer AGCTCGTTACCCGGGGATC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byW.B. Pollock
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For an explanation of the cytochrome c expression system, see "Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition." by Pollock et al. PMID 9558351
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBTR1 was a gift from Gary Pielak (Addgene plasmid # 22468 ; http://n2t.net/addgene:22468 ; RRID:Addgene_22468)
For your References section:Stability and apoptotic activity of recombinant human cytochrome c. Olteanu A, Patel CN, Dedmon MM, Kennedy S, Linhoff MW, Minder CM, Potts PR, Deshmukh M, Pielak GJ. Biochem Biophys Res Commun. 2003 Dec 19. 312(3):733-40. 10.1016/j.bbrc.2003.10.182 PubMed 14680826
Map uploaded by the depositor.