pBTR(hCc)
(Plasmid #61026)

• Purpose: Expresses horse holocytochrome c in E. coli
• Depositing Lab: Gary Pielak
• Publication: Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4.

Backbone

• Vector backbone: pBTR(C102T)
• Backbone manufacturer: Multiple labs
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing laboratory recommends using bacterial strain BL21(DE3) for protein expression
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: horse cytochrome c
• Species: Equus*
• Mutation: *see comments
• GenBank ID: NP_001157486.1
• Entrez Gene: CYCS
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 Reverse
• 3′ sequencing primer: M13 (-21) Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Notes from depositor: CYC3 encodes the enzyme, which we call heme lysase in the paper, that covalently attaches the heme to the apocytochrome c. That is, the plasmid has BOTH the CYC3 gene and the structural gene for horse cytochrome c. The horse cytochrome C sequence has been codon optimized.