pMSP2N2
(Plasmid #29520)

• Depositing Lab: Stephen Sligar
• Publication: Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):843-8.

Backbone

• Vector backbone: pET 28a
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MSP2N2
• Alt name: membrane scaffold protein;
• Alt name: apolipoprotein A-1
• Alt name: APOA1
• Species: synthetic gene
• Insert Size (bp): 1280
• Mutation: tandem MSP; His-tag on N-terminus followed by spacer sequence and TEV protease site
• Tag / Fusion Protein:
  • 7-His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Addgene Notes:
  • Sequence with T7-term primer
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is distributed in DH5alpha cells. For protein expression use BL-21(DE3)gold; high oxygenation level is required.