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(Plasmid #29520)


Item Catalog # Description Quantity Price (USD)
Plasmid 29520 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pET 28a
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    membrane scaffold protein;
  • Alt name
    apolipoprotein A-1
  • Alt name
  • Species
    synthetic gene
  • Insert Size (bp)
  • Mutation
    tandem MSP; His-tag on N-terminus followed by spacer sequence and TEV protease site
  • Tag / Fusion Protein
    • 7-His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is distributed in DH5alpha cells. For protein expression use BL-21(DE3)gold; high oxygenation level is required.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP2N2 was a gift from Stephen Sligar (Addgene plasmid # 29520 ; ; RRID:Addgene_29520)
  • For your References section:

    Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers. Grinkova YV, Denisov IG, Sligar SG. Protein Eng Des Sel. 2010 Nov . 23(11):843-8. 10.1093/protein/gzq060 PubMed 20817758