|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29520||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepET 28a
- Backbone size w/o insert (bp) 5300
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Alt namemembrane scaffold protein;
Alt nameapolipoprotein A-1
Insert Size (bp)1280
Mutationtandem MSP; His-tag on N-terminus followed by spacer sequence and TEV protease site
/ Fusion Protein
- 7-His tag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid is distributed in DH5alpha cells. For protein expression use BL-21(DE3)gold; high oxygenation level is required.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSP2N2 was a gift from Stephen Sligar (Addgene plasmid # 29520 ; http://n2t.net/addgene:29520 ; RRID:Addgene_29520)
For your References section:Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers. Grinkova YV, Denisov IG, Sligar SG. Protein Eng Des Sel. 2010 Nov . 23(11):843-8. 10.1093/protein/gzq060 PubMed 20817758