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Addgene

pMSP1E2
(Plasmid #20063)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20063 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET 28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL-21(DE3); high oxygenation level
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MSP1E2
  • Alt name
    membrane scaffold protein
  • Alt name
    APOA1
  • Species
    synthetic gene
  • Insert Size (bp)
    770
  • Mutation
    "extended" MSP1; contains repeats of helices 4 and 5
  • Entrez Gene
    APOA1 (a.k.a. AMYLD3, HPALP2, apo(a))
  • Tag / Fusion Protein
    • 6-His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1E2 was a gift from Stephen Sligar (Addgene plasmid # 20063 ; http://n2t.net/addgene:20063 ; RRID:Addgene_20063)
  • For your References section:

    Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. Denisov IG, Grinkova YV, Lazarides AA, Sligar SG. J Am Chem Soc. 2004 Mar 24. 126(11):3477-87. 10.1021/ja0393574 PubMed 15025475