Cell Migration Consortium (CMC) Plasmids
This resource is a collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited plasmids useful for cell migration research at Addgene.
CMC investigators who have deposited plasmids include:
- Joan Brugge, Harvard Medical School
- Klaus Hahn, UNC
- Richard Hynes, MIT
- Rick Horwitz, University of Virginia
- Denise Montell, University of California, Santa Barbara
- Martin Schwartz, Yale University
- Stephen Sligar, University of Illinois
- Ron Vale, University of California, San Francisco
Browse Plasmids
| ID | Plasmid | Gene/Insert | Vector Type | Backbone | Mutations | Tags | PI | |
|---|---|---|---|---|---|---|---|---|
| 12150 | pTriEx-RhoA FLARE.sc Biosensor WT | RhoA Biosensor (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | 6xHis | Hahn | ||
| 12151 | pTriEx-RhoA FLARE.sc Biosensor Q63L | RhoA Biosensor Q63L (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | RhoA Q63L (dominant-positive mutation) | 6xHis | Hahn | |
| 12152 | pTriEx-RhoA FLARE.sc Biosensor T19N | RhoA Biosensor T19N (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | RhoA T19N (dominant-negative mutation)* | 6xHis | Hahn | |
| 12153 | pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor | RhoA Biosensor (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-Sin-Puro-Tet-CMV | 6XHis | Hahn | ||
| 12597 | pET23-CBD-(-PP)-EGFP | Cdc42 Biosensor (Homo sapiens) | Bacterial Expression | pET23 | F271C | EGFP, 6xHis | Hahn | |
| 12598 | pET23-CBD-(-PP)-EGFP-H246,249D | Cdc42 Biosensor Control (Homo sapiens) | Bacterial Expression | pET23 | EGFP, 6xHis | Hahn | ||
| 12599 | pcDNA3-EGFP-Cdc42(wt) | EGFP-Cdc42 (WT) (Homo sapiens) | Mammalian Expression | pcDNA3 | EGFP | Hahn | ||
| 12600 | pcDNA3-EGFP-Cdc42(Q61L) | EGFP-Cdc42 (Q61L) (Homo sapiens) | Mammalian Expression | pcDNA3 | Q61L | EGFP | Hahn | |
| 12601 | pcDNA3-EGFP-Cdc42(T17N) | EGFP-Cdc42 (T17N) (Homo sapiens) | Mammalian Expression | pcDNA3 | T17N | EGFP | Hahn | |
| 12602 | pBabe-Puro-RhoA FLARE.sc Biosensor | RhoA Biosensor (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-Puro-CMV | Hahn | |||
| 13657 | pCMV5 mouse src K295R Y527F | src K295R Y527F (Mus musculus) | Mammalian Expression | pCMV5 * | K295R Y527F. Dominant negative mutant. | Brugge | ||
| 13658 | pLNCX mouse src | src (Mus musculus) | Mammalian Expression, Retroviral | pLNCX | Brugge | |||
| 13659 | pLNCX chick src K295R | src K295R (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | K295R. Kinase dead mutant. | Brugge | ||
| 13660 | pLNCX chick src Y527F | src Y527F (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | Y527F. Constitutive active mutant. | Brugge | ||
| 13661 | pLNCX chick src E378G | src E378G (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | E378G. Constitutive active mutant. | Brugge | ||
| 13662 | pLNCX chick src Y416F | src Y416F (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | Y416F | Brugge | ||
| 13663 | pCMV5 mouse src | src (Mus musculus) | Mammalian Expression | pCMV5 * | Myr | Brugge | ||
| 13665 | pLNCX chick src | Src (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | Brugge | |||
| 13719 | pcDNA3-EGFP-Rac1(wt) | EGFP-Rac1(wt) (Homo sapiens) | Mammalian Expression | pcDNA3 | EGFP | Hahn | ||
| 13720 | pcDNA3-EGFP-Rac1(Q61L) | EGFP-Rac1(Q61L) (Homo sapiens) | Mammalian Expression | pcDNA3 | EGFP | Hahn | ||
| 13721 | pcDNA3-EGFP-Rac1(T17N) | EGFP-Rac1(T17N) (Homo sapiens) | Mammalian Expression | pcDNA3 | EGFP | Hahn | ||
| 13722 | pET23-PBD(65-150)-N-Cys | PBD(65-150)-N-Cys (Homo sapiens) | Bacterial Expression | pET23 | Met-Cys-Gly-PBD(65-150)- | 6xHis | Hahn | |
| 13723 | pcDNA3-PBD-EYFP | PBD-EYFP (Homo sapiens) | Mammalian Expression | pcDNA3 | p21 binding domain of human Pak1(amino acids 65-150) | EYFP | Hahn | |
| 14056 | p-rlf-1 | Rat Fibronectin (Rattus norvegicus) | Mammalian Expression | pGEM-2 | Hynes | |||
| 14057 | pSR270 | Rat Fibronectin (Rattus norvegicus) | Mammalian Expression | pGEM-2 | Hynes | |||
| 14059 | pEIIIA | Rat Fibronectin EIIIA (Rattus norvegicus) | Mammalian Expression | pGEM-2 | EcoRI linkers added to TaqI to HinfI fragment | Hynes | ||
| 14060 | pEIIIB | Rat Fibronectin EIIIB (Rattus norvegicus) | Mammalian Expression | pGEM-2 | EcoRI linkers were attached to HindIII - HinfI fragment. | Hynes | ||
| 14061 | pchfn01 | Chicken Fibronectin (Gallus gallus) | Mammalian Expression | pGEM-2 | Hynes | |||
| 14062 | pcFNE3A (chick fibronectin E3A) | Chicken Fibronectin EIIIA (Gallus gallus) | Mammalian Expression | pGem-3 | Blunted BstE2-AvaII fragment was inserted into blunted BamHI-HincII sites | Hynes | ||
| 14063 | pcFN-C (chick fibronectin) | Chicken Fibronectin (Gallus gallus) | Mammalian Expression | pGem-3 | DraI - EcoRI fragment inserted in HincII-EcoRI sites | Hynes | ||
| 14064 | pcFNE3B (chick fibronectin E3B) | Chicken Fibronectin EIIIB (Gallus gallus) | Mammalian Expression | pGem-3 | Hynes | |||
| 14065 | 7iBi89 (rat fibronectin) | Rat Fibronectin 7iBi89 (Rattus norvegicus) | Mammalian Expression | pBAGH.Sv | Insert was cut out of pGEM3 with SalI and SacI and blunt end cloned into BamHI sites. | Hynes | ||
| 14066 | p1DT (chicken beta 1 integrin) | Chicken Beta 1 Integrin (Gallus gallus) | Mammalian Expression | pECE | Hynes | |||
| 14067 | pCINTBeta1 | Chicken Beta 1 Integrin (Gallus gallus) | Mammalian Expression | pGEM-2 | PstI-BglI fragment was blunt end cloned and EcoRI linkers added and inserted in the EcoRI site. | Hynes | ||
| 14069 | Beta Nu | Drosphila Beta Nu Integrin (Drosophila melanogaster) | Bacterial Expression | PBluescript II SK- | Hynes | |||
| 14070 | Alt AlphaPS3 | Drosphila Alt Alpha PS3 Integrin (Drosophila melanogaster) | Bacterial Expression | pCR-Script SK+ | Hynes | |||
| 14071 | Comp AlphaPS3 | Drosphila Comp Alpha PS3 Integrin (Drosophila melanogaster) | Bacterial Expression | PBluescript II SK- | Hynes | |||
| 14097 | pMA5.1 (mouse integrin alpha 5 probe) | Mouse Alpha 5 Integrin (Mus musculus) | Bacterial Expression | pBluescript II SK+ | Hynes | |||
| 14098 | pMA4.1 (mouse integrin alpha 4 probe) | Mouse Alpha 4 Integrin (Mus musculus) | Bacterial Expression | pBluescript II SK+ | Hynes | |||
| 14099 | pCR-BluntII-TOPO-RhoC | Human RhoC (Homo sapiens) | Bacterial Expression | PCR-BluntII-TOPO | Hynes | |||
| 14107 | pET_Unc653_GFP_His | Unc104 (1-653) (Caenorhabditis elegans) | Bacterial Expression | pET17 | GFP, His | Vale | ||
| 14119 | pMIG-RhoC | Human RhoC (Homo sapiens) | Retroviral | pMIG | Hynes | |||
| 14120 | pMIG-N19RhoA | N19 Human RhoA (Homo sapiens) | Retroviral | pMIG | Site directed mutagenesis of Thr to Asn at Codon 19 | Myc | Hynes | |
| 14127 | pMINTBeta1 | Mouse Beta 1 Integrin (Mus musculus) | Mammalian Expression | pGEM 1 | Hynes | |||
| 14549 | pMSCV neo WT Akt1 | Akt1 (Mus musculus) | Mammalian Expression, Retroviral | pMSCV neo | HA tag | Brugge | ||
| 14550 | pMSCV neo KD Akt1 | Akt1 KD (Mus musculus) | Mammalian Expression, Retroviral | pMSCV neo | Kinase Dead Mutant: K179M | HA tag | Brugge | |
| 14551 | pBabe WT Akt2 | Akt2 (Homo sapiens) | Mammalian Expression, Retroviral | pBABE-puro | HA tag | Brugge | ||
| 14553 | pC.HA Vav1 | Vav1 (Homo sapiens) | Mammalian Expression | pC.HA | HA | Brugge | ||
| 14554 | pC.HA Vav2 | Vav2 (Homo sapiens) | Mammalian Expression | pC.HA | HA | Brugge | ||
| 14555 | pC.HA Vav3 | Vav3 (Mus musculus) | Mammalian Expression | pC.HA | HA | Brugge | ||
| 14557 | pMX Vav1 uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | GFP | Brugge | ||
| 14558 | pMX Vav1 Y3F uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F (Y142, Y160, Y174) in DH domain | GFP | Brugge | |
| 14559 | pMX Vav1 Y3F/DH* (L213Q) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F (Y142, Y160, Y174)/DH* (L213Q) | GFP | Brugge | |
| 14560 | pMX Vav1 Y3F/CR* (C529S) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F/CR* (C529S) | GFP | Brugge | |
| 14562 | pMX Vav1 Y3F/NSH3* (P651L) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F/NSH3* (P651L) | GFP | Brugge | |
| 14563 | pMX Vav1 Y3F/Grb2bindb* (P657A) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F/Grb2bindb* (P657A) | GFP | Brugge | |
| 14564 | pMX Vav1 Y3F/PH* (W495L) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | PH* (W495L) | GFP | Brugge | |
| 14565 | pMX Vav1 Y3F/SH2* (R696K) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F/SH2* (R696K) | GFP | Brugge | |
| 14566 | pMX Vav1 Y3F/CSH3* (P833L) uGFP | Vav1 (Homo sapiens) | Mammalian Expression, Retroviral | pMX-uGFP | Y3F/CSH3* (P833L) | GFP | Brugge | |
| 14567 | pMX GFP Rac G12V | Rac1 G12V (Homo sapiens) | Mammalian Expression, Retroviral | pMX | G12V | EGFP | Brugge | |
| 14568 | pMX GFP cdc42 Q61L | cdc42 Q61L (Homo sapiens) | Mammalian Expression, Retroviral | pMX | Q61L | EGFP | Brugge | |
| 14570 | JP1520 PIK3CA WT HA | PIK3CA | Mammalian Expression, Retroviral | JP1520 | HA tag | Brugge | ||
| 14571 | JP1520 PIK3CA E545K HA | PIK3CA E545K (Bos taurus) | Mammalian Expression, Retroviral | JP1520 | E545K | HA tag | Brugge | |
| 14572 | JP1520 PIK3CA H1047R HA | PIK3CA H1047R (Bos taurus) | Mammalian Expression, Retroviral | JP1520 | H1047R | HA tag | Brugge | |
| 14578 | pLNCX chick v-src | v-Src (Gallus gallus) | Mammalian Expression, Retroviral | pLNCX | Brugge | |||
| 15126 | pC.HA | Mammalian Expression | pCF1 | HA | Brugge | |||
| 15211 | Flag-ECFP-Paxillin | paxillin (Gallus gallus) | Mammalian Expression | pcDNA3 | Flag, ECFP | Horwitz | ||
| 15212 | Flag-Paxillin | Paxillin (Gallus gallus) | Mammalian Expression | pcDNA3 | Flag | Horwitz | ||
| 15219 | pET17_K560_GFP_His | Human neuronal kinesin heavy chain (Homo sapiens) | Bacterial Expression | pET17b | GFP, His | Vale | ||
| 15223 | Flag-ECFP-GIT1 | G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) | Mammalian Expression | pCDNA3 | flag, ECFP | Horwitz | ||
| 15225 | GIT1A-FLAG | G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) | Mammalian Expression | pEGFP-N1 | flag | Horwitz | ||
| 15226 | pEGFP-GIT1 | G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) | Mammalian Expression | PEGFP-C1 | L425V, E476K and L583P | GFP | Horwitz | |
| 15233 | Paxillin-pEGFP | paxillin (Gallus gallus) | Mammalian Expression | pEGFP-N3 | GFP | Horwitz | ||
| 15234 | Flag-betaPIXa | beta-PIXa (Mus musculus) | Mammalian Expression | pcDNA3 | Flag | Horwitz | ||
| 15235 | Flag-ECFP-betaPIXa | beta-PIXa (Mus musculus) | Mammalian Expression | pcDNA3 | Flag, ECFP | Horwitz | ||
| 15238 | Alpha 5 integrin-GFP | alpha 5 integrin (Homo sapiens) | Mammalian Expression | pEGFP-N3 | EGFP | Horwitz | ||
| 15239 | pWBC | Human neuronal kinesin heavy chain (Homo sapiens) | Bacterial Expression | pBS SK- | Vale | |||
| 15247 | GST-RBD | Rhotekin (Mus musculus) | Bacterial Expression | pGEX-2T | amino acids 7-89 (Rho binding domain) | GST | Schwartz | |
| 15284 | pXPE | ubiquitous kinesin heavy chain (Homo sapiens) | Bacterial Expression | pSP72 | The insert is missing the 1st 180 bp of 5' untranslated region of the original cDNA clone | Vale | ||
| 15312 | pMT-EGFP-Actin5c | Actin5c (Drosophila melanogaster) | Insect Expression | pMT-V5/His'B' | His, EGFP | Vale | ||
| 15682 | pBABE YAP1 | YAP (Homo sapiens) | Mammalian Expression, Retroviral | pBABE puro | Flag | Brugge | ||
| 16290 | LAMP1-GFP | LAMP1/RE72002 (Drosophila melanogaster) | Insect Expression | pAc5.1/V5-His B | EGFP | Vale | ||
| 17235 | pMKO shRNA Bim | Bim shRNA (Homo sapiens) | Mammalian Expression, Retroviral, RNAi | pMKO.1 | Brugge | |||
| 17236 | pMKO shRNA Bmf | Bmf shRNA (Homo sapiens) | Mammalian Expression, Retroviral, RNAi | pMKO.1 | Brugge | |||
| 17237 | pMKO shRNA Bad | Bad shRNA (Homo sapiens) | Mammalian Expression, Retroviral, RNAi | pMKO.1 | Brugge | |||
| 17239 | pBabe HA human Bmf | Bmf (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-neo | HA | Brugge | ||
| 17240 | pBabe human Bmf | Bmf (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-neo | Brugge | |||
| 17241 | pBabe mouse Bmf | Bmf (Mus musculus) | Mammalian Expression, Retroviral | pBabe-neo | Brugge | |||
| 17242 | pBabe 3xFLAG human Bmf | Bmf (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-neo | 3xFLAG | Brugge | ||
| 17243 | pBabe 3xFLAG mouse Bmf | Bmf (Mus musculus) | Mammalian Expression, Retroviral | pBabe-neo | 3xFLAG | Brugge | ||
| 17244 | pcDNA3 mouse Bmf | Bmf (Mus musculus) | Mammalian Expression | pcDNA3 | Brugge | |||
| 17245 | pLNCX myr Akt1 | Akt1 (Mus musculus) | Mammalian Expression, Retroviral | pLNCX | Constitutively active | Myr | Brugge | |
| 17493 | pBabe puro c-met WT | c-Met (Mus musculus) | Mammalian Expression, Retroviral | pBabe puro | Brugge | |||
| 17580 | Doughnut (DNT) | dnt (Drosophila melanogaster) | Bacterial Expression | pCRII | Montell | |||
| 17581 | hs-lambda btl | lambda-btl (Drosophila melanogaster) | Insect Expression | pCaSpeR-hs | Montell | |||
| 17582 | hs casper RhoL-V12 | RhoL (Drosophila melanogaster) | Insect Expression | pCaSpeR-hs | V12 | Montell | ||
| 17583 | hs casper RhoL-N17 | RhoL (Drosophila melanogaster) | Insect Expression | pCaSpeR-hs | N17 | Montell | ||
| 17584 | pcDNA3-taiman | taiman (Drosophila melanogaster) | Mammalian Expression | pcDNA3 | Montell | |||
| 17585 | pCaSpeR-hs-taiman | taiman (Drosophila melanogaster) | Insect Expression | pCaSpeR-hs | Montell | |||
| 17586 | pBluescript KS(-) otk | otk (Drosophila melanogaster) | Bacterial Expression | pBluescript KS(-) | Montell | |||
| 17587 | CG4845/pUAST-HA | CG4845 (Drosophila melanogaster) | Insect Expression | pUAST | HA | Montell | ||
| 17588 | CG4845/pUAST | CG4845 (Drosophila melanogaster) | Insect Expression | pUAST | Montell | |||
| 17589 | CG4845/pMT/V5-HisB | CG4845 (Drosophila melanogaster) | Insect Expression | pMT/V5-His B | 6x His, V5 | Montell | ||
| 17788 | pUS200 | Cytochrome P450cam | Bacterial Expression | pEMBL8+ | Sligar | |||
| 18840 | CyPET-Arf6 | CyPET-Arf6 (Homo sapiens) | Mammalian Expression | pEF4-myc-HisA | The sequence for the fluorescent protein CyPET was inserted into human Arf6 between amino acid residues 143 and 144, adding 6 amino acid linkers. | Schwartz | ||
| 18841 | YPET-GGA3 | YPET-GGA3 (Homo sapiens) | Mammalian Expression | pEF4-myc-HisA | The fluorescent protein YPET was added to the amino terminus of amino acids 148-303 from GGA3. | myc, His6 | Schwartz | |
| 19744 | MSCV FLIP FF | oligo targeting Firefly luciferase | Mammalian Expression, Retroviral, RNAi, Cre/Lox | MSCV FLIP | Hynes | |||
| 19745 | MSCV FLIP p53 | oligo targeting p53 (Mus musculus) | Mammalian Expression, Retroviral, RNAi, Cre/Lox | MSCV FLIP | Hynes | |||
| 19746 | MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN) | oligo targeting p53 and PTEN (Mus musculus) | Mammalian Expression, Retroviral, RNAi, Cre/Lox | MSCV FLIPi | Hynes | |||
| 19748 | MSCV FLIPi P2G_Thy1.1 p53 | oligo targeting p53 (Mus musculus) | Mammalian Expression, Retroviral, RNAi, Cre/Lox | MSCV FLIPi | Hynes | |||
| 19749 | MSCV FLIPi P2G_Thy1.1 PTEN | oligo targeting PTEN (Mus musculus) | Mammalian Expression, Retroviral, RNAi, Cre/Lox | MSCV FLIPi | Hynes | |||
| 19750 | MSCV P2Gm FF | oligo targeting Firefly luciferase | Mammalian Expression, Retroviral, RNAi | MSCV P2Gm | Hynes | |||
| 19751 | MSCV P2Gm p53 | oligo targeting p53 (Mus musculus) | Mammalian Expression, Retroviral, RNAi | MSCV P2Gm | Hynes | |||
| 19752 | pLB2 CAG P2Gm | Mammalian Expression, Mouse Targeting, Lentiviral, RNAi | LB2 | Hynes | ||||
| 19753 | pLB2 Ubi-FLIP FF | oligo targeting Firefly luciferase | Mammalian Expression, Mouse Targeting, Lentiviral, RNAi, Cre/Lox | LB2 FLIP | Hynes | |||
| 20058 | pMB413a | Sperm Whale Myoglobin | Bacterial Expression | pUC19 | Sligar | |||
| 20059 | pVS45 | rat hepatic cytochrome b5 (Rattus norvegicus) | Bacterial Expression | pUC13 | Sligar | |||
| 20061 | pMSP1D1 | MSP1D1 | Bacterial Expression | pET 28a | deletion mutant (1-11) of MSP1; His-tag on N-terminus followed by spacer sequence and TEV protease site | 7-His tag | Sligar | |
| 20062 | pMSP1E1 | MSP1E1 | Bacterial Expression | pET 28a | "extended" MSP1; contains repeat of helix 4 | 6-His | Sligar | |
| 20063 | pMSP1E2 | MSP1E2 | Bacterial Expression | pET 28a | "extended" MSP1; contains repeats of helices 4 and 5 | 6-His | Sligar | |
| 20064 | pMSP1E3 | MSP1E3 | Bacterial Expression | pET 28a | "extended" MSP1; contains repeats of helices 4, 5 and 6 | 6-His | Sligar | |
| 20066 | pMSP1E3D1 | MSP1E3D1 (Other) | Bacterial Expression | pET 28a | "extended" MSP1D1; contains repeats of helices 4, 5 and 6;N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site | 7-His | Sligar | |
| 22007 | pTriEx-mVenus-PA-Rac1 | PA-Rac1 (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His, mVenus | Hahn | |
| 22008 | pBabe-TetCMV-puro-mVenus-PA-Rac1 | mVenus-PA-Rac1 (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22017 | pTriEx-mVenus-PA-Rac1-T17N | PA-Rac1-T17N (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutation T17N. | 6X His, mVenus | Hahn | |
| 22018 | pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N | mVenus-PA-Rac1-T17N (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Rac1 starts at I4 and contains mutation T17N. | Hahn | ||
| 22021 | pTriEx-mVenus-PA-Rac1-C450A | PA-Rac1-C450A (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | LOV domain contains the C450A mutation.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His, mVenus | Hahn | |
| 22022 | pTriEx-mPA-GFP-PA-Rac1 | PA-Rac1 (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His, mPA-GFP | Hahn | |
| 22023 | pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A | mVenus-PA-Rac1-C450A (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | The LOV domain contains the mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22024 | pTriEx-PA-Rac1 | PA-Rac1 (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His | Hahn | |
| 22025 | pTriEx-PA-Rac1-C450A | PA-Rac1-C450A (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | LOV domain contains mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His | Hahn | |
| 22026 | pTriEx-PA-Rac1-I539E | PA-Rac1-I539E (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | LOV domain contains mutation I539E.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His | Hahn | |
| 22027 | pTriEx-mCherry-PA-Rac1 | PA-Rac1 (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H (results in the photoactivatable analogue of Rac1) | 6X His, mCherry | Hahn | |
| 22028 | pTriEx-mCherry-PA-Rac1-C450A | PA-Rac1-C450A (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | The LOV domain contains the mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His, mCherry | Hahn | |
| 22029 | pTriEx-mCherry-PA-Rac1-T17N | PA-Rac1-T17N (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutation T17N. | 6X His, mCherry | Hahn | |
| 22030 | pTriEx-mCerulean-PA-Rac1 | PA-Rac1 (Homo sapiens) | Mammalian Expression, Bacterial Expression, Insect Expression | pTriEx | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | 6X His, mCerulean | Hahn | |
| 22031 | pBabe-TetCMV-puro-mCerulean-PA-Rac1 | mCerulean-PA-Rac1 (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22032 | pBabe-TetCMV-puro-mPA-GFP-PA-Rac1 | mPA-GFP-PA-Rac1 (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22033 | pBabe-TetCMV-puro-mVenus-LOV | mVenus-LOV | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Insert length based on Venus (720bp) + LOV (450bp) | Hahn | ||
| 22034 | pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M | mVenus-PA-Rac1-C450M (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | The LOV domain contains the mutation C450M.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22035 | pBabe-TetCMV-puro-mCherry-PA-Rac1 | mCherry-PA-Rac1 (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-TetCMV-puro | Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. | Hahn | ||
| 22380 | SRS-MTBD(22/19), AB(monomer), wt | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc, His | Vale | ||
| 22381 | SRS-MTBD(26/19) AB(monomer), wt | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc | Vale | ||
| 22382 | SRS-MTBD(26/19)ux, dimer, no myc | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | His | Vale | ||
| 22384 | SRS-MTBD(89/82)-ABux | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc, His | Vale | ||
| 22385 | SRS-MTBD(89/82)-ux, dimer, no myc | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | Vale | |||
| 22386 | SRS-MTBD(26/19) AB, ux | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc | Vale | ||
| 22388 | SRS-MTBD(19/19), dimer, no myc, ux | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | Vale | |||
| 22389 | SRS-MTBD(19/19), AB(monomer), ux | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | Myc, His | Vale | ||
| 22390 | SRS-MTBD(22/19), ux, dimer, no myc | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | Vale | |||
| 22391 | SRS-MTBD(22/19), AB(monomer), ux | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | Vale | |||
| 22392 | SRS-MTBD(22/19)wt, dimer, plus myc tag | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc | Vale | ||
| 22393 | SRS-MTBD(89/82)-AB wt | Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus) | Bacterial Expression | pET42a | myc, His | Vale | ||
| 22699 | MSCV P2Gm +SwaI | miR30 | Mammalian Expression, Retroviral, RNAi | MSCV2.2 with WPRE cloned into ClaI site is the base vector | Hynes | |||
| 22779 | pCyPet-C1 | CyPet | Mammalian Expression | pCyPet-C1-Control | Control for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have CyPet. | Hahn | ||
| 22780 | pYpet-C1 | Ypet | Mammalian Expression | pYpet-C1-Control | Control for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have Ypet. | Hahn | ||
| 22781 | pYpet-PBD | PAK binding domain (Homo sapiens) | Mammalian Expression | pYpet-C1 | Ypet | Hahn | ||
| 22782 | pCyPet-Rac1(WT) | Rac1 (wt) (Homo sapiens) | Mammalian Expression | pCyPet-C1 | CyPet | Hahn | ||
| 22783 | pCyPet-Rac1(Q61L) | Rac1 (Q61L) (Homo sapiens) | Mammalian Expression | pCyPet-C1 | Rac1 Q61L. | CyPet | Hahn | |
| 22784 | pCyPet-Rac1(T17N) | Rac1 (T17N) (Homo sapiens) | Mammalian Expression | pCyPet-C1 | Rac1 T17N. | CyPet | Hahn | |
| 24166 | CLM RP KGF Mut 1 | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A | GFP, 6XHis | Vale | |
| 24282 | pMT-GFP-Spindly | Spindly (Drosophila melanogaster) | Insect Expression | pMT-GW | GFP | Vale | ||
| 24283 | pMT-GFP-p150Dynactin | p150 Dynactin (Drosophila melanogaster) | Insect Expression | pMT-GW | GFP | Vale | ||
| 24284 | pMT-Mad2-GFP | Mad2 (Drosophila melanogaster) | Insect Expression | pMT-WG | GFP | Vale | ||
| 24285 | pMT-Aurora B-GFP | Aurora B Kinase (Drosophila melanogaster) | Insect Expression | pMT-WG | GFP | Vale | ||
| 24286 | pMT-Pav-GFP | Pavarotti (Drosophila melanogaster) | Insect Expression | pMT-GFP | GFP | Vale | ||
| 24287 | pMT-Diaphanous-GFP | Diaphanous (Drosophila melanogaster) | Insect Expression | pMT-WG | GFP | Vale | ||
| 24288 | pAc-mCh-Tub | Alpha-Tubulin (Drosophila melanogaster) | Insect Expression | pAc-mCherryW | mCherry | Vale | ||
| 24430 | CLM RP HTR | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A | 6XHis | Vale | |
| 24431 | RP hk339-GFP | Kinesin WT (Homo sapiens) | Bacterial Expression | pET17b | monomeric kinesin | GFP, 6XHis | Vale | |
| 24432 | A337C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C | GFP, 6XHis | Vale | |
| 24433 | Y344C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C | GFP, 6XHis | Vale | |
| 24434 | A337C/Y344C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y344C | GFP, 6XHis | Vale | |
| 24435 | K222C/E334C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E334C | GFP, 6XHis | Vale | |
| 24436 | L4C/S330C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A,, L4C/S330C | GFP, 6XHis | Vale | |
| 24437 | L4C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, L4C | GFP, 6XHis | Vale | |
| 24438 | K222C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C | GFP, 6XHis | Vale | |
| 24439 | S330C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S/C421A, "S330C" | GFP, 6XHis | Vale | |
| 24441 | K339CLM S330C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S, S330C | GFP, 6XHis | Vale | |
| 24442 | K343CLM A337C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S, A337C | GFP, 6XHis | Vale | |
| 24443 | K420CLM RP | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only | Vale | ||
| 24444 | 560HTR | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | 6XHis | Vale | ||
| 24447 | KRP95 | Kinesin II | Bacterial Expression | pET17b | GFP | Vale | ||
| 24448 | KRP85 | Kinesin II | Bacterial Expression | pET17b | GFP | Vale | ||
| 24449 | K339CLM G234A/215/347 | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E347C | His | Vale | |
| 24451 | K349CLM-6His 215/347 | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C | His | Vale | |
| 24454 | K347CLM G234A/43/347 | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E347C | His | Vale | |
| 24460 | K560CLM T324C | Kinesin (Homo sapiens) | Bacterial Expression | pET17b | C7S/C65A/C168A/C174S/C294A/C330S T324C/C421A | His | Vale | |
| 24469 | U371-HTR | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | Contains only the kinesin's motor head domain (amino acids 1-371). | His | Vale | |
| 24470 | U653-HTR | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | His | Vale | ||
| 24472 | U371-GFP ILtoEK | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | Contains only the kinesin's motor head fused to GFP (1-371) I362E/L365K | GFP | Vale | |
| 24473 | U653-GFP ILtoEK | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | I362E/L365K (neck destabilization mutant) | GFP | Vale | |
| 24476 | U371-GFP | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | Contains only the kinesin's motor head fused to GFP (1-371) | GFP | Vale | |
| 24477 | U653-GFP | Unc104 (Caenorhabditis elegans) | Bacterial Expression | pET17 | GFP | Vale | ||
| 24507 | NCD-GFP | NCD (Drosophila melanogaster) | Bacterial Expression | pET17b | GFP, His | Vale | ||
| 24508 | NK-1 GFP | Drosophila NCD, human kinesin (Homo sapiens) | Bacterial Expression | pET17b | NK-1 is a chimera where the entire superfamily-conserved catalytic domain (aa 348-667) of NCD was substituted for the equivalent domain of inesin (aa 8-322). GFP is mutant (S65T) | GFP, His | Vale | |
| 24533 | pBabe-Ypet-PBD | PAK binding domain (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-sin-tet-CMV-puro | Ypet | Hahn | ||
| 24534 | pBabe-CyPet-Rac1 | Rac1 (Homo sapiens) | Mammalian Expression, Retroviral | pBabe-sin-tet-CMV-puro | CyPet | Hahn | ||
| 25919 | pEGFP-FRB | FRB (Homo sapiens) | Mammalian Expression | pEGFP-C1 | Hahn | |||
| 25920 | pCherry-FRB | FRB (Homo sapiens) | Mammalian Expression | pCherry | mCherry | Hahn | ||
| 25926 | myc-Rapr-FAK | RapR-FAK (Mus musculus) | Mammalian Expression | pRc/CMV | Myc | Hahn | ||
| 25927 | myc-Rapr-FAK-YM | RapR-FAK (Mus musculus) | Mammalian Expression | pRc/CMV | This myc-RapR-FAK-YM plasmid contains FAK mutations Y180A and M183A. (The sequence data uploaded to addgene is based on wt). | Myc | Hahn | |
| 25928 | pEGFP-RapR-FAK-YM | RapR-FAK (Homo sapiens) | Mammalian Expression | pEGFP-C1 | This pEGFP-RapR-FAK-YM plasmid contains the FAK mutations Y180A and M183A. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. | EGFP | Hahn | |
| 25929 | pEGFP-RapR-FAK-YM-KD | RapR-FAK (Homo sapiens) | Mammalian Expression | pEGFP-C1 | This pEGFP-RapR-FAK-YM-KD plasmid contains the FAK mutations Y180A and M183A, as well as the kinase dead mutation D546R. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. | EGFP | Hahn | |
| 25930 | pEGFP-RapR-FAK-YM-Y397F | RapR-FAK (Homo sapiens) | Mammalian Expression | pEGFP-C1 | This pEGFP-RapR-FAK-YM-Y397F plasmid contains the FAK mutations Y180A and M183A, as well as the mutation Y397F. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. | EGFP | Hahn | |
| 25933 | pUSE-RapR-Src-myc | RapR-Src (Mus musculus) | Mammalian Expression | pUSE | Myc | Hahn | ||
| 25934 | pUSE-RapR-Src(KD)-myc | RapR-Src (Mus musculus) | Mammalian Expression | pUSE | This pUSE-RapR-Src(KD)-myc plasmid contains the kinase inactive mutation D388R. The uploaded sequence data is based on wt. | Hahn | ||
| 25935 | pCMV5-Flag-RapR-p38 | RapR-p38 (Homo sapiens) | Mammalian Expression | pCMV5 | Flag | Hahn | ||
| 26019 | VinculinTS | Vinculin tension sensor | Mammalian Expression | pcDNA3.1 | Schwartz | |||
| 26020 | Tailless VinculinTS | tailless vinculinTS | Mammalian Expression | pcDNA3.1 | Schwartz | |||
| 26021 | TS module | Tension Sensor module | Mammalian Expression | pcDNA3.1 | Schwartz | |||
| 27300 | Vinculin-venus | Vinculin (Gallus gallus) | Mammalian Expression | pcDNA3.1(+) | Venus A206K | Schwartz |
Related Resource
Expression constructs for studying protein structures can also be obtained from the Protein Production and Analysis Facility at the Burnham Institute.