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cmc-logo.gif Cell Migration Consortium (CMC) Plasmids


This resource is a collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited plasmids useful for cell migration research at Addgene.

CMC investigators who have deposited plasmids include:

Browse Plasmids

ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI
12150pTriEx-RhoA FLARE.sc Biosensor WTRhoA Biosensor (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx6xHis Hahn
12151pTriEx-RhoA FLARE.sc Biosensor Q63LRhoA Biosensor Q63L (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx RhoA Q63L (dominant-positive mutation) 6xHis Hahn
12152pTriEx-RhoA FLARE.sc Biosensor T19NRhoA Biosensor T19N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx RhoA T19N (dominant-negative mutation)* 6xHis Hahn
12153pBabe-Sin-Puro-Tet-RhoA FLARE.sc BiosensorRhoA Biosensor (Homo sapiens)Mammalian Expression, RetroviralpBabe-Sin-Puro-Tet-CMV6XHis Hahn
12597pET23-CBD-(-PP)-EGFPCdc42 Biosensor (Homo sapiens)Bacterial ExpressionpET23 F271C EGFP, 6xHis Hahn
12598pET23-CBD-(-PP)-EGFP-H246,249DCdc42 Biosensor Control (Homo sapiens)Bacterial ExpressionpET23EGFP, 6xHis Hahn
12599pcDNA3-EGFP-Cdc42(wt)EGFP-Cdc42 (WT) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP Hahn
12600pcDNA3-EGFP-Cdc42(Q61L)EGFP-Cdc42 (Q61L) (Homo sapiens)Mammalian ExpressionpcDNA3 Q61L EGFP Hahn
12601pcDNA3-EGFP-Cdc42(T17N)EGFP-Cdc42 (T17N) (Homo sapiens)Mammalian ExpressionpcDNA3 T17N EGFP Hahn
12602pBabe-Puro-RhoA FLARE.sc BiosensorRhoA Biosensor (Homo sapiens)Mammalian Expression, RetroviralpBabe-Puro-CMV Hahn
13657pCMV5 mouse src K295R Y527Fsrc K295R Y527F (Mus musculus)Mammalian ExpressionpCMV5 * K295R Y527F. Dominant negative mutant. Brugge
13658pLNCX mouse srcsrc (Mus musculus)Mammalian Expression, RetroviralpLNCX Brugge
13659pLNCX chick src K295Rsrc K295R (Gallus gallus)Mammalian Expression, RetroviralpLNCX K295R. Kinase dead mutant. Brugge
13660pLNCX chick src Y527Fsrc Y527F (Gallus gallus)Mammalian Expression, RetroviralpLNCX Y527F. Constitutive active mutant. Brugge
13661pLNCX chick src E378Gsrc E378G (Gallus gallus)Mammalian Expression, RetroviralpLNCX E378G. Constitutive active mutant. Brugge
13662pLNCX chick src Y416Fsrc Y416F (Gallus gallus)Mammalian Expression, RetroviralpLNCX Y416F Brugge
13663pCMV5 mouse srcsrc (Mus musculus)Mammalian ExpressionpCMV5 *Myr Brugge
13665pLNCX chick srcSrc (Gallus gallus)Mammalian Expression, RetroviralpLNCX Brugge
13719pcDNA3-EGFP-Rac1(wt)EGFP-Rac1(wt) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP Hahn
13720pcDNA3-EGFP-Rac1(Q61L)EGFP-Rac1(Q61L) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP Hahn
13721pcDNA3-EGFP-Rac1(T17N)EGFP-Rac1(T17N) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP Hahn
13722pET23-PBD(65-150)-N-CysPBD(65-150)-N-Cys (Homo sapiens)Bacterial ExpressionpET23 Met-Cys-Gly-PBD(65-150)- 6xHis Hahn
13723pcDNA3-PBD-EYFPPBD-EYFP (Homo sapiens)Mammalian ExpressionpcDNA3 p21 binding domain of human Pak1(amino acids 65-150) EYFP Hahn
14056p-rlf-1Rat Fibronectin (Rattus norvegicus)Mammalian ExpressionpGEM-2 Hynes
14057pSR270Rat Fibronectin (Rattus norvegicus)Mammalian ExpressionpGEM-2 Hynes
14059pEIIIARat Fibronectin EIIIA (Rattus norvegicus)Mammalian ExpressionpGEM-2 EcoRI linkers added to TaqI to HinfI fragment Hynes
14060pEIIIBRat Fibronectin EIIIB (Rattus norvegicus)Mammalian ExpressionpGEM-2 EcoRI linkers were attached to HindIII - HinfI fragment. Hynes
14061pchfn01Chicken Fibronectin (Gallus gallus)Mammalian ExpressionpGEM-2 Hynes
14062pcFNE3A (chick fibronectin E3A)Chicken Fibronectin EIIIA (Gallus gallus)Mammalian ExpressionpGem-3 Blunted BstE2-AvaII fragment was inserted into blunted BamHI-HincII sites Hynes
14063pcFN-C (chick fibronectin)Chicken Fibronectin (Gallus gallus)Mammalian ExpressionpGem-3 DraI - EcoRI fragment inserted in HincII-EcoRI sites Hynes
14064pcFNE3B (chick fibronectin E3B)Chicken Fibronectin EIIIB (Gallus gallus)Mammalian ExpressionpGem-3 Hynes
140657iBi89 (rat fibronectin)Rat Fibronectin 7iBi89 (Rattus norvegicus)Mammalian ExpressionpBAGH.Sv Insert was cut out of pGEM3 with SalI and SacI and blunt end cloned into BamHI sites. Hynes
14066p1DT (chicken beta 1 integrin)Chicken Beta 1 Integrin (Gallus gallus)Mammalian ExpressionpECE Hynes
14067pCINTBeta1Chicken Beta 1 Integrin (Gallus gallus)Mammalian ExpressionpGEM-2 PstI-BglI fragment was blunt end cloned and EcoRI linkers added and inserted in the EcoRI site. Hynes
14069Beta NuDrosphila Beta Nu Integrin (Drosophila melanogaster)Bacterial ExpressionPBluescript II SK- Hynes
14070Alt AlphaPS3Drosphila Alt Alpha PS3 Integrin (Drosophila melanogaster)Bacterial ExpressionpCR-Script SK+ Hynes
14071Comp AlphaPS3Drosphila Comp Alpha PS3 Integrin (Drosophila melanogaster)Bacterial ExpressionPBluescript II SK- Hynes
14097pMA5.1 (mouse integrin alpha 5 probe)Mouse Alpha 5 Integrin (Mus musculus)Bacterial ExpressionpBluescript II SK+ Hynes
14098pMA4.1 (mouse integrin alpha 4 probe)Mouse Alpha 4 Integrin (Mus musculus)Bacterial ExpressionpBluescript II SK+ Hynes
14099pCR-BluntII-TOPO-RhoCHuman RhoC (Homo sapiens)Bacterial ExpressionPCR-BluntII-TOPO Hynes
14107pET_Unc653_GFP_HisUnc104 (1-653) (Caenorhabditis elegans)Bacterial ExpressionpET17GFP, His Vale
14119pMIG-RhoCHuman RhoC (Homo sapiens)RetroviralpMIG Hynes
14120pMIG-N19RhoAN19 Human RhoA (Homo sapiens)RetroviralpMIG Site directed mutagenesis of Thr to Asn at Codon 19 Myc Hynes
14127pMINTBeta1Mouse Beta 1 Integrin (Mus musculus)Mammalian ExpressionpGEM 1 Hynes
14549pMSCV neo WT Akt1Akt1 (Mus musculus)Mammalian Expression, RetroviralpMSCV neoHA tag Brugge
14550pMSCV neo KD Akt1Akt1 KD (Mus musculus)Mammalian Expression, RetroviralpMSCV neo Kinase Dead Mutant: K179M HA tag Brugge
14551pBabe WT Akt2Akt2 (Homo sapiens)Mammalian Expression, RetroviralpBABE-puroHA tag Brugge
14553pC.HA Vav1Vav1 (Homo sapiens)Mammalian ExpressionpC.HAHA Brugge
14554pC.HA Vav2Vav2 (Homo sapiens)Mammalian ExpressionpC.HAHA Brugge
14555pC.HA Vav3Vav3 (Mus musculus)Mammalian ExpressionpC.HAHA Brugge
14557pMX Vav1 uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFPGFP Brugge
14558pMX Vav1 Y3F uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F (Y142, Y160, Y174) in DH domain GFP Brugge
14559pMX Vav1 Y3F/DH* (L213Q) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F (Y142, Y160, Y174)/DH* (L213Q) GFP Brugge
14560pMX Vav1 Y3F/CR* (C529S) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F/CR* (C529S) GFP Brugge
14562pMX Vav1 Y3F/NSH3* (P651L) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F/NSH3* (P651L) GFP Brugge
14563pMX Vav1 Y3F/Grb2bindb* (P657A) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F/Grb2bindb* (P657A) GFP Brugge
14564pMX Vav1 Y3F/PH* (W495L) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP PH* (W495L) GFP Brugge
14565pMX Vav1 Y3F/SH2* (R696K) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F/SH2* (R696K) GFP Brugge
14566pMX Vav1 Y3F/CSH3* (P833L) uGFPVav1 (Homo sapiens)Mammalian Expression, RetroviralpMX-uGFP Y3F/CSH3* (P833L) GFP Brugge
14567pMX GFP Rac G12VRac1 G12V (Homo sapiens)Mammalian Expression, RetroviralpMX G12V EGFP Brugge
14568pMX GFP cdc42 Q61Lcdc42 Q61L (Homo sapiens)Mammalian Expression, RetroviralpMX Q61L EGFP Brugge
14570JP1520 PIK3CA WT HAPIK3CAMammalian Expression, RetroviralJP1520HA tag Brugge
14571JP1520 PIK3CA E545K HAPIK3CA E545K (Bos taurus)Mammalian Expression, RetroviralJP1520 E545K HA tag Brugge
14572JP1520 PIK3CA H1047R HAPIK3CA H1047R (Bos taurus)Mammalian Expression, RetroviralJP1520 H1047R HA tag Brugge
14578pLNCX chick v-srcv-Src (Gallus gallus)Mammalian Expression, RetroviralpLNCX Brugge
15126pC.HAMammalian ExpressionpCF1HA Brugge
15211Flag-ECFP-Paxillinpaxillin (Gallus gallus)Mammalian ExpressionpcDNA3Flag, ECFP Horwitz
15212Flag-PaxillinPaxillin (Gallus gallus)Mammalian ExpressionpcDNA3Flag Horwitz
15219pET17_K560_GFP_HisHuman neuronal kinesin heavy chain (Homo sapiens)Bacterial ExpressionpET17bGFP, His Vale
15223Flag-ECFP-GIT1G protein coupled receptor kinase-interacting protein 1 (Homo sapiens)Mammalian ExpressionpCDNA3flag, ECFP Horwitz
15225GIT1A-FLAGG protein coupled receptor kinase-interacting protein 1 (Homo sapiens)Mammalian ExpressionpEGFP-N1flag Horwitz
15226pEGFP-GIT1G protein coupled receptor kinase-interacting protein 1 (Homo sapiens)Mammalian ExpressionPEGFP-C1 L425V, E476K and L583P GFP Horwitz
15233Paxillin-pEGFPpaxillin (Gallus gallus)Mammalian ExpressionpEGFP-N3GFP Horwitz
15234Flag-betaPIXabeta-PIXa (Mus musculus)Mammalian ExpressionpcDNA3Flag Horwitz
15235Flag-ECFP-betaPIXabeta-PIXa (Mus musculus)Mammalian ExpressionpcDNA3Flag, ECFP Horwitz
15238Alpha 5 integrin-GFPalpha 5 integrin (Homo sapiens)Mammalian ExpressionpEGFP-N3EGFP Horwitz
15239pWBCHuman neuronal kinesin heavy chain (Homo sapiens)Bacterial ExpressionpBS SK- Vale
15247GST-RBDRhotekin (Mus musculus)Bacterial ExpressionpGEX-2T amino acids 7-89 (Rho binding domain) GST Schwartz
15284pXPEubiquitous kinesin heavy chain (Homo sapiens)Bacterial ExpressionpSP72 The insert is missing the 1st 180 bp of 5' untranslated region of the original cDNA clone Vale
15312pMT-EGFP-Actin5cActin5c (Drosophila melanogaster)Insect ExpressionpMT-V5/His'B'His, EGFP Vale
15682pBABE YAP1YAP (Homo sapiens)Mammalian Expression, RetroviralpBABE puroFlag Brugge
16290LAMP1-GFPLAMP1/RE72002 (Drosophila melanogaster)Insect ExpressionpAc5.1/V5-His BEGFP Vale
17235pMKO shRNA BimBim shRNA (Homo sapiens)Mammalian Expression, Retroviral, RNAipMKO.1 Brugge
17236pMKO shRNA BmfBmf shRNA (Homo sapiens)Mammalian Expression, Retroviral, RNAipMKO.1 Brugge
17237pMKO shRNA BadBad shRNA (Homo sapiens)Mammalian Expression, Retroviral, RNAipMKO.1 Brugge
17239pBabe HA human BmfBmf (Homo sapiens)Mammalian Expression, RetroviralpBabe-neoHA Brugge
17240pBabe human BmfBmf (Homo sapiens)Mammalian Expression, RetroviralpBabe-neo Brugge
17241pBabe mouse BmfBmf (Mus musculus)Mammalian Expression, RetroviralpBabe-neo Brugge
17242pBabe 3xFLAG human BmfBmf (Homo sapiens)Mammalian Expression, RetroviralpBabe-neo3xFLAG Brugge
17243pBabe 3xFLAG mouse BmfBmf (Mus musculus)Mammalian Expression, RetroviralpBabe-neo3xFLAG Brugge
17244pcDNA3 mouse BmfBmf (Mus musculus)Mammalian ExpressionpcDNA3 Brugge
17245pLNCX myr Akt1Akt1 (Mus musculus)Mammalian Expression, RetroviralpLNCX Constitutively active Myr Brugge
17493pBabe puro c-met WTc-Met (Mus musculus)Mammalian Expression, RetroviralpBabe puro Brugge
17580Doughnut (DNT)dnt (Drosophila melanogaster)Bacterial ExpressionpCRII Montell
17581hs-lambda btllambda-btl (Drosophila melanogaster)Insect ExpressionpCaSpeR-hs Montell
17582hs casper RhoL-V12RhoL (Drosophila melanogaster)Insect ExpressionpCaSpeR-hs V12 Montell
17583hs casper RhoL-N17RhoL (Drosophila melanogaster)Insect ExpressionpCaSpeR-hs N17 Montell
17584pcDNA3-taimantaiman (Drosophila melanogaster)Mammalian ExpressionpcDNA3 Montell
17585pCaSpeR-hs-taimantaiman (Drosophila melanogaster)Insect ExpressionpCaSpeR-hs Montell
17586pBluescript KS(-) otkotk (Drosophila melanogaster)Bacterial ExpressionpBluescript KS(-) Montell
17587CG4845/pUAST-HACG4845 (Drosophila melanogaster)Insect ExpressionpUASTHA Montell
17588CG4845/pUASTCG4845 (Drosophila melanogaster)Insect ExpressionpUAST Montell
17589CG4845/pMT/V5-HisBCG4845 (Drosophila melanogaster)Insect ExpressionpMT/V5-His B6x His, V5 Montell
17788pUS200Cytochrome P450camBacterial ExpressionpEMBL8+ Sligar
18840CyPET-Arf6CyPET-Arf6 (Homo sapiens)Mammalian ExpressionpEF4-myc-HisA The sequence for the fluorescent protein CyPET was inserted into human Arf6 between amino acid residues 143 and 144, adding 6 amino acid linkers. Schwartz
18841YPET-GGA3YPET-GGA3 (Homo sapiens)Mammalian ExpressionpEF4-myc-HisA The fluorescent protein YPET was added to the amino terminus of amino acids 148-303 from GGA3. myc, His6 Schwartz
19744MSCV FLIP FFoligo targeting Firefly luciferaseMammalian Expression, Retroviral, RNAi, Cre/LoxMSCV FLIP Hynes
19745MSCV FLIP p53oligo targeting p53 (Mus musculus)Mammalian Expression, Retroviral, RNAi, Cre/LoxMSCV FLIP Hynes
19746MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)oligo targeting p53 and PTEN (Mus musculus)Mammalian Expression, Retroviral, RNAi, Cre/LoxMSCV FLIPi Hynes
19748MSCV FLIPi P2G_Thy1.1 p53oligo targeting p53 (Mus musculus)Mammalian Expression, Retroviral, RNAi, Cre/LoxMSCV FLIPi Hynes
19749MSCV FLIPi P2G_Thy1.1 PTENoligo targeting PTEN (Mus musculus)Mammalian Expression, Retroviral, RNAi, Cre/LoxMSCV FLIPi Hynes
19750MSCV P2Gm FFoligo targeting Firefly luciferaseMammalian Expression, Retroviral, RNAiMSCV P2Gm Hynes
19751MSCV P2Gm p53oligo targeting p53 (Mus musculus)Mammalian Expression, Retroviral, RNAiMSCV P2Gm Hynes
19752pLB2 CAG P2GmMammalian Expression, Mouse Targeting, Lentiviral, RNAiLB2 Hynes
19753pLB2 Ubi-FLIP FFoligo targeting Firefly luciferaseMammalian Expression, Mouse Targeting, Lentiviral, RNAi, Cre/LoxLB2 FLIP Hynes
20058pMB413aSperm Whale MyoglobinBacterial ExpressionpUC19 Sligar
20059pVS45rat hepatic cytochrome b5 (Rattus norvegicus)Bacterial ExpressionpUC13 Sligar
20060pMSP1MSP1Bacterial ExpressionpET 28a synthetic gene of deletion mutant (1-43) of human Apolipoprotein A-1; contains N-terminal 6-His tag and Factor X site; the codons optimized for E. coli expression 6-His Sligar
20061pMSP1D1MSP1D1Bacterial ExpressionpET 28a deletion mutant (1-11) of MSP1; His-tag on N-terminus followed by spacer sequence and TEV protease site 7-His tag Sligar
20062pMSP1E1MSP1E1Bacterial ExpressionpET 28a "extended" MSP1; contains repeat of helix 4 6-His Sligar
20063pMSP1E2MSP1E2Bacterial ExpressionpET 28a "extended" MSP1; contains repeats of helices 4 and 5 6-His Sligar
20064pMSP1E3MSP1E3Bacterial ExpressionpET 28a "extended" MSP1; contains repeats of helices 4, 5 and 6 6-His Sligar
20066pMSP1E3D1MSP1E3D1 (Other)Bacterial ExpressionpET 28a "extended" MSP1D1; contains repeats of helices 4, 5 and 6;N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site 7-His Sligar
22007pTriEx-mVenus-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His, mVenus Hahn
22008pBabe-TetCMV-puro-mVenus-PA-Rac1mVenus-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22017pTriEx-mVenus-PA-Rac1-T17NPA-Rac1-T17N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutation T17N. 6X His, mVenus Hahn
22018pBabe-TetCMV-puro-mVenus-PA-Rac1-T17NmVenus-PA-Rac1-T17N (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro Rac1 starts at I4 and contains mutation T17N. Hahn
22021pTriEx-mVenus-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx LOV domain contains the C450A mutation.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His, mVenus Hahn
22022pTriEx-mPA-GFP-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His, mPA-GFP Hahn
22023pBabe-TetCMV-puro-mVenus-PA-Rac1-C450AmVenus-PA-Rac1-C450A (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro The LOV domain contains the mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22024pTriEx-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His Hahn
22025pTriEx-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx LOV domain contains mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His Hahn
22026pTriEx-PA-Rac1-I539EPA-Rac1-I539E (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx LOV domain contains mutation I539E.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His Hahn
22027pTriEx-mCherry-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutations Q61L, E91H and N92H (results in the photoactivatable analogue of Rac1) 6X His, mCherry Hahn
22028pTriEx-mCherry-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx The LOV domain contains the mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His, mCherry Hahn
22029pTriEx-mCherry-PA-Rac1-T17NPA-Rac1-T17N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutation T17N. 6X His, mCherry Hahn
22030pTriEx-mCerulean-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 6X His, mCerulean Hahn
22031pBabe-TetCMV-puro-mCerulean-PA-Rac1mCerulean-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22032pBabe-TetCMV-puro-mPA-GFP-PA-Rac1mPA-GFP-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22033pBabe-TetCMV-puro-mVenus-LOVmVenus-LOVMammalian Expression, RetroviralpBabe-TetCMV-puro Insert length based on Venus (720bp) + LOV (450bp) Hahn
22034pBabe-TetCMV-puro-mVenus-PA-Rac1-C450MmVenus-PA-Rac1-C450M (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro The LOV domain contains the mutation C450M.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22035pBabe-TetCMV-puro-mCherry-PA-Rac1mCherry-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralpBabe-TetCMV-puro Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. Hahn
22380SRS-MTBD(22/19), AB(monomer), wtSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc, His Vale
22381SRS-MTBD(26/19) AB(monomer), wtSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc Vale
22382SRS-MTBD(26/19)ux, dimer, no mycSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42aHis Vale
22384SRS-MTBD(89/82)-ABuxSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc, His Vale
22385SRS-MTBD(89/82)-ux, dimer, no mycSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42a Vale
22386SRS-MTBD(26/19) AB, uxSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc Vale
22388SRS-MTBD(19/19), dimer, no myc, uxSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42a Vale
22389SRS-MTBD(19/19), AB(monomer), uxSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42aMyc, His Vale
22390SRS-MTBD(22/19), ux, dimer, no mycSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42a Vale
22391SRS-MTBD(22/19), AB(monomer), uxSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42a Vale
22392SRS-MTBD(22/19)wt, dimer, plus myc tagSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc Vale
22393SRS-MTBD(89/82)-AB wtSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein (Mus musculus)Bacterial ExpressionpET42amyc, His Vale
22699MSCV P2Gm +SwaImiR30Mammalian Expression, Retroviral, RNAiMSCV2.2 with WPRE cloned into ClaI site is the base vector Hynes
22779pCyPet-C1CyPetMammalian ExpressionpCyPet-C1-Control Control for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have CyPet. Hahn
22780pYpet-C1YpetMammalian ExpressionpYpet-C1-Control Control for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have Ypet. Hahn
22781pYpet-PBDPAK binding domain (Homo sapiens)Mammalian ExpressionpYpet-C1Ypet Hahn
22782pCyPet-Rac1(WT)Rac1 (wt) (Homo sapiens)Mammalian ExpressionpCyPet-C1CyPet Hahn
22783pCyPet-Rac1(Q61L)Rac1 (Q61L) (Homo sapiens)Mammalian ExpressionpCyPet-C1 Rac1 Q61L. CyPet Hahn
22784pCyPet-Rac1(T17N)Rac1 (T17N) (Homo sapiens)Mammalian ExpressionpCyPet-C1 Rac1 T17N. CyPet Hahn
24166CLM RP KGF Mut 1Kinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A GFP, 6XHis Vale
24282pMT-GFP-SpindlySpindly (Drosophila melanogaster)Insect ExpressionpMT-GWGFP Vale
24283pMT-GFP-p150Dynactinp150 Dynactin (Drosophila melanogaster)Insect ExpressionpMT-GWGFP Vale
24284pMT-Mad2-GFPMad2 (Drosophila melanogaster)Insect ExpressionpMT-WGGFP Vale
24285pMT-Aurora B-GFPAurora B Kinase (Drosophila melanogaster)Insect ExpressionpMT-WGGFP Vale
24286pMT-Pav-GFPPavarotti (Drosophila melanogaster)Insect ExpressionpMT-GFPGFP Vale
24287pMT-Diaphanous-GFPDiaphanous (Drosophila melanogaster)Insect ExpressionpMT-WGGFP Vale
24288pAc-mCh-TubAlpha-Tubulin (Drosophila melanogaster)Insect ExpressionpAc-mCherryWmCherry Vale
24430CLM RP HTRKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A 6XHis Vale
24431RP hk339-GFPKinesin WT (Homo sapiens)Bacterial ExpressionpET17b monomeric kinesin GFP, 6XHis Vale
24432A337CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C GFP, 6XHis Vale
24433Y344CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C GFP, 6XHis Vale
24434A337C/Y344CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y344C GFP, 6XHis Vale
24435K222C/E334CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E334C GFP, 6XHis Vale
24436L4C/S330CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A,, L4C/S330C GFP, 6XHis Vale
24437L4CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, L4C GFP, 6XHis Vale
24438K222CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C GFP, 6XHis Vale
24439S330CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S/C421A, "S330C" GFP, 6XHis Vale
24441K339CLM S330CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S, S330C GFP, 6XHis Vale
24442K343CLM A337CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S, A337C GFP, 6XHis Vale
24443K420CLM RPKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only Vale
24444560HTRKinesin (Homo sapiens)Bacterial ExpressionpET17b6XHis Vale
24447KRP95Kinesin IIBacterial ExpressionpET17bGFP Vale
24448KRP85Kinesin IIBacterial ExpressionpET17bGFP Vale
24449K339CLM G234A/215/347Kinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E347C His Vale
24451K349CLM-6His 215/347Kinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C His Vale
24454K347CLM G234A/43/347Kinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E347C His Vale
24460K560CLM T324CKinesin (Homo sapiens)Bacterial ExpressionpET17b C7S/C65A/C168A/C174S/C294A/C330S T324C/C421A His Vale
24469U371-HTRUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17 Contains only the kinesin's motor head domain (amino acids 1-371). His Vale
24470U653-HTRUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17His Vale
24472U371-GFP ILtoEKUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17 Contains only the kinesin's motor head fused to GFP (1-371) I362E/L365K GFP Vale
24473U653-GFP ILtoEKUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17 I362E/L365K (neck destabilization mutant) GFP Vale
24476U371-GFPUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17 Contains only the kinesin's motor head fused to GFP (1-371) GFP Vale
24477U653-GFPUnc104 (Caenorhabditis elegans)Bacterial ExpressionpET17GFP Vale
24507NCD-GFPNCD (Drosophila melanogaster)Bacterial ExpressionpET17bGFP, His Vale
24508NK-1 GFPDrosophila NCD, human kinesin (Homo sapiens)Bacterial ExpressionpET17b NK-1 is a chimera where the entire superfamily-conserved catalytic domain (aa 348-667) of NCD was substituted for the equivalent domain of inesin (aa 8-322). GFP is mutant (S65T) GFP, His Vale
24533pBabe-Ypet-PBDPAK binding domain (Homo sapiens)Mammalian Expression, RetroviralpBabe-sin-tet-CMV-puroYpet Hahn
24534pBabe-CyPet-Rac1Rac1 (Homo sapiens)Mammalian Expression, RetroviralpBabe-sin-tet-CMV-puroCyPet Hahn
25919pEGFP-FRBFRB (Homo sapiens)Mammalian ExpressionpEGFP-C1 Hahn
25920pCherry-FRBFRB (Homo sapiens)Mammalian ExpressionpCherrymCherry Hahn
25926myc-Rapr-FAKRapR-FAK (Mus musculus)Mammalian ExpressionpRc/CMVMyc Hahn
25927myc-Rapr-FAK-YMRapR-FAK (Mus musculus)Mammalian ExpressionpRc/CMV This myc-RapR-FAK-YM plasmid contains FAK mutations Y180A and M183A. (The sequence data uploaded to addgene is based on wt). Myc Hahn
25928pEGFP-RapR-FAK-YMRapR-FAK (Homo sapiens)Mammalian ExpressionpEGFP-C1 This pEGFP-RapR-FAK-YM plasmid contains the FAK mutations Y180A and M183A. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. EGFP Hahn
25929pEGFP-RapR-FAK-YM-KDRapR-FAK (Homo sapiens)Mammalian ExpressionpEGFP-C1 This pEGFP-RapR-FAK-YM-KD plasmid contains the FAK mutations Y180A and M183A, as well as the kinase dead mutation D546R. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. EGFP Hahn
25930pEGFP-RapR-FAK-YM-Y397FRapR-FAK (Homo sapiens)Mammalian ExpressionpEGFP-C1 This pEGFP-RapR-FAK-YM-Y397F plasmid contains the FAK mutations Y180A and M183A, as well as the mutation Y397F. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human. EGFP Hahn
25933pUSE-RapR-Src-mycRapR-Src (Mus musculus)Mammalian ExpressionpUSEMyc Hahn
25934pUSE-RapR-Src(KD)-mycRapR-Src (Mus musculus)Mammalian ExpressionpUSE This pUSE-RapR-Src(KD)-myc plasmid contains the kinase inactive mutation D388R. The uploaded sequence data is based on wt. Hahn
25935pCMV5-Flag-RapR-p38RapR-p38 (Homo sapiens)Mammalian ExpressionpCMV5Flag Hahn
26019VinculinTSVinculin tension sensorMammalian ExpressionpcDNA3.1 Schwartz
26020Tailless VinculinTStailless vinculinTSMammalian ExpressionpcDNA3.1 Schwartz
26021TS moduleTension Sensor moduleMammalian ExpressionpcDNA3.1 Schwartz
27300Vinculin-venusVinculin (Gallus gallus)Mammalian ExpressionpcDNA3.1(+)Venus A206K Schwartz

Related Resource

Expression constructs for studying protein structures can also be obtained from the Protein Production and Analysis Facility at the Burnham Institute.