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Klaus Hahn Lab Plasmids


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Title Authors Publication
Spatiotemporal dynamics of RhoA activity in migrating cells. Pertz O, Hodgson L, Klemke RL, Hahn KM Nature. 2006 Apr 20. 440(7087):1069-72.
Activation of endogenous Cdc42 visualized in living cells. Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM Science. 2004 Sep 10. 305(5690):1615-9.
Localized Rac activation dynamics visualized in living cells. Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn KM Science. 2000 Oct 13. 290(5490):333-7.
A genetically encoded photoactivatable Rac controls the motility of living cells. Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM Nature. 2009 Sep 3. 461(7260):104-8.
Coordination of Rho GTPase activities during cell protrusion. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell A, Johnson GL, Hahn KM, Danuser G Nature. 2009 Sep 3. 461(7260):99-103.
Engineered allosteric activation of kinases in living cells. Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM Nat Biotechnol. 2010 Jun 27. ():.
pET23-CBD (unpublished)
Rational design of a ligand-controlled protein conformational switch. Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn KM, Dokholyan NV Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 10.1073/pnas.1218319110. Epub 2013 Apr 8.
An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Rao MV, Chu PH, Hahn KM, Zaidel-Bar R Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24.
A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013.
Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13.
LOVTRAP: an optogenetic system for photoinduced protein dissociation. Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
Engineering extrinsic disorder to control protein activity in living cells. Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM Science. 2016 Dec 16;354(6318):1441-1444.
FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Hodgson L, Spiering D, Sabouri-Ghomi M, Dagliyan O, DerMardirossian C, Danuser G, Hahn KM Nat Chem Biol. 2016 Aug 8. doi: 10.1038/nchembio.2145.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12150pTriEx-RhoA FLARE.sc Biosensor WTRhoA Biosensor (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionSpatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20. 440(7087):1069-72. pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
12151pTriEx-RhoA FLARE.sc Biosensor Q63LRhoA Biosensor Q63L (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionSpatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20. 440(7087):1069-72. pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
12152pTriEx-RhoA FLARE.sc Biosensor T19NRhoA Biosensor T19N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionSpatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20. 440(7087):1069-72. pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
12153pBabe-Sin-Puro-Tet-RhoA FLARE.sc BiosensorRhoA Biosensor (Homo sapiens)Mammalian Expression, RetroviralSpatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20. 440(7087):1069-72. pBabe-Sin-Puro-Tet-CMV RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
12597pET23-CBD-(-PP)-EGFPCdc42 Biosensor (Homo sapiens)Bacterial ExpressionActivation of endogenous Cdc42 visualized in living cells. Science. 2004 Sep 10. 305(5690):1615-9. pET23 WAS IMD2, SCNX, THC, THC1, WASP, WASPA Add to Cart
12598pET23-CBD-(-PP)-EGFP-H246,249DCdc42 Biosensor Control (Homo sapiens)Bacterial ExpressionActivation of endogenous Cdc42 visualized in living cells. Science. 2004 Sep 10. 305(5690):1615-9. pET23 Add to Cart
12599pcDNA3-EGFP-Cdc42(wt)EGFP-Cdc42 (WT) (Homo sapiens)Mammalian ExpressionActivation of endogenous Cdc42 visualized in living cells. Science. 2004 Sep 10. 305(5690):1615-9. pcDNA3 CDC42 CDC42Hs, G25K, TKS Add to Cart
12600pcDNA3-EGFP-Cdc42(Q61L)EGFP-Cdc42 (Q61L) (Homo sapiens)Mammalian ExpressionActivation of endogenous Cdc42 visualized in living cells. Science. 2004 Sep 10. 305(5690):1615-9. pcDNA3 CDC42 CDC42Hs, G25K, TKS Add to Cart
12601pcDNA3-EGFP-Cdc42(T17N)EGFP-Cdc42 (T17N) (Homo sapiens)Mammalian ExpressionActivation of endogenous Cdc42 visualized in living cells. Science. 2004 Sep 10. 305(5690):1615-9. pcDNA3 CDC42 CDC42Hs, G25K, TKS Add to Cart
12602pBabe-Puro-RhoA FLARE.sc BiosensorRhoA Biosensor (Homo sapiens)Mammalian Expression, RetroviralSpatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20. 440(7087):1069-72. pBabe-Puro-CMV RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
13719pcDNA3-EGFP-Rac1(wt)EGFP-Rac1(wt) (Homo sapiens)Mammalian ExpressionLocalized Rac activation dynamics visualized in living cells. Science. 2000 Oct 13. 290(5490):333-7. pcDNA3 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
13720pcDNA3-EGFP-Rac1(Q61L)EGFP-Rac1(Q61L) (Homo sapiens)Mammalian ExpressionLocalized Rac activation dynamics visualized in living cells. Science. 2000 Oct 13. 290(5490):333-7. pcDNA3 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
13721pcDNA3-EGFP-Rac1(T17N)EGFP-Rac1(T17N) (Homo sapiens)Mammalian ExpressionLocalized Rac activation dynamics visualized in living cells. Science. 2000 Oct 13. 290(5490):333-7. pcDNA3 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
13722pET23-PBD(65-150)-N-CysPBD(65-150)-N-Cys (Homo sapiens)Bacterial ExpressionLocalized Rac activation dynamics visualized in living cells. Science. 2000 Oct 13. 290(5490):333-7. pET23 PAK1 PAKalpha Add to Cart
13723pcDNA3-PBD-EYFPPBD-EYFP (Homo sapiens)Mammalian ExpressionLocalized Rac activation dynamics visualized in living cells. Science. 2000 Oct 13. 290(5490):333-7. pcDNA3 PAK1 PAKalpha Add to Cart
22007pTriEx-mVenus-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22008pBabe-TetCMV-puro-mVenus-PA-Rac1mVenus-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22017pTriEx-mVenus-PA-Rac1-T17NPA-Rac1-T17N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22018pBabe-TetCMV-puro-mVenus-PA-Rac1-T17NmVenus-PA-Rac1-T17N (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22021pTriEx-mVenus-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22022pTriEx-mPA-GFP-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22023pBabe-TetCMV-puro-mVenus-PA-Rac1-C450AmVenus-PA-Rac1-C450A (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22024pTriEx-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22025pTriEx-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22026pTriEx-PA-Rac1-I539EPA-Rac1-I539E (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22027pTriEx-mCherry-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22028pTriEx-mCherry-PA-Rac1-C450APA-Rac1-C450A (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22029pTriEx-mCherry-PA-Rac1-T17NPA-Rac1-T17N (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22030pTriEx-mCerulean-PA-Rac1PA-Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22031pBabe-TetCMV-puro-mCerulean-PA-Rac1mCerulean-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22032pBabe-TetCMV-puro-mPA-GFP-PA-Rac1mPA-GFP-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22033pBabe-TetCMV-puro-mVenus-LOVmVenus-LOVMammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro Add to Cart
22034pBabe-TetCMV-puro-mVenus-PA-Rac1-C450MmVenus-PA-Rac1-C450M (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22035pBabe-TetCMV-puro-mCherry-PA-Rac1mCherry-PA-Rac1 (Homo sapiens)Mammalian Expression, RetroviralA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. pBabe-TetCMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22779pCyPet-C1CyPetMammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pCyPet-C1-Control Add to Cart
22780pYpet-C1YpetMammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pYpet-C1-Control Add to Cart
22781pYpet-PBDPAK binding domain (Homo sapiens)Mammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pYpet-C1 Add to Cart
22782pCyPet-Rac1(WT)Rac1 (wt) (Homo sapiens)Mammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pCyPet-C1 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22783pCyPet-Rac1(Q61L)Rac1 (Q61L) (Homo sapiens)Mammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pCyPet-C1 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
22784pCyPet-Rac1(T17N)Rac1 (T17N) (Homo sapiens)Mammalian ExpressionCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pCyPet-C1 RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
24533pBabe-Ypet-PBDPAK binding domain (Homo sapiens)Mammalian Expression, RetroviralCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pBabe-sin-tet-CMV-puro PAK1 PAKalpha Add to Cart
24534pBabe-CyPet-Rac1Rac1 (Homo sapiens)Mammalian Expression, RetroviralCoordination of Rho GTPase activities during cell protrusion. Nature. 2009 Sep 3. 461(7260):99-103. pBabe-sin-tet-CMV-puro RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
25919pEGFP-FRBFRB (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pEGFP-C1 MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS Add to Cart
25920pCherry-FRBFRB (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pCherry MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS Add to Cart
25926myc-Rapr-FAKRapR-FAK (Mus musculus)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pRc/CMV Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
25927myc-Rapr-FAK-YMRapR-FAK (Mus musculus)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pRc/CMV Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
25928pEGFP-RapR-FAK-YMRapR-FAK (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pEGFP-C1 Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
25929pEGFP-RapR-FAK-YM-KDRapR-FAK (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pEGFP-C1 Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
25930pEGFP-RapR-FAK-YM-Y397FRapR-FAK (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pEGFP-C1 Ptk2 FAK, FRNK, Fadk, mKIAA4203, p125FAK Add to Cart
25933pUSE-RapR-Src-mycRapR-Src (Mus musculus)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pUSE Src AW259666, pp60c-src Add to Cart
25934pUSE-RapR-Src(KD)-mycRapR-Src (Mus musculus)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pUSE Fkbp1a FKBP12, Fkbp, Fkbp1 Add to Cart
25935pCMV5-Flag-RapR-p38RapR-p38 (Homo sapiens)Mammalian ExpressionEngineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010 Jun 27. ():. pCMV5 Mapk14 CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha Add to Cart
30311pET23-CBDCBD (Homo sapiens)Bacterial Expression pET23-CBD (unpublished) pET23 WAS IMD2, SCNX, THC, THC1, WASP, WASPA Add to Cart
30312pGFP(C3)-VinculinVinculin (Mus musculus)Mammalian Expression pET23-CBD (unpublished) pGFP-C3 Vcl 9430097D22, AA571387, AI462105, AW545629 Add to Cart
45381pUSE-Src-YF-UniRapR-mCerulean-mycSrc-YF-UniRapR-mCerulean-myc (Mus musculus)Mammalian ExpressionRational design of a ligand-controlled protein conformational switch. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 10.1073/pnas.1218319110. Epub 2013 Apr 8. pUSE Add to Cart
45382pUSE-Src-KD-UniRapR-mCerulean-mycSrc-KD-UniRapR-mCerulean-myc (Mus musculus)Mammalian ExpressionRational design of a ligand-controlled protein conformational switch. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 10.1073/pnas.1218319110. Epub 2013 Apr 8. pUSE Add to Cart
45509Tol2 Krt4 Src (YF)-uniRapR-ceruleanSrc (YF)-uniRapR-ceruleanzebrafish expressionRational design of a ligand-controlled protein conformational switch. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 10.1073/pnas.1218319110. Epub 2013 Apr 8. pT2KHspGGFF (Asakawa K, Kawakami K. Dev Growth Differ. 2008) Add to Cart
45583GFP-CA-mDia1Constitutively active mDia1 (CA-mDia1) (Mus musculus)Mammalian ExpressionAn optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. pEGFP-C1 Diaph1 D18Wsu154e, Dia1, Diap1, Drf1, p140mDia Add to Cart
53095PA-DAD-wtLOV(wt)-6aa-DAD (Mus musculus)Mammalian Expression, Bacterial Expression, Insect ExpressionAn optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light. pTriEx4 Add to Cart
53096PA-DAD-darkLOV(dark)-6aa-DAD (Mus musculus)Mammalian Expression, Bacterial Expression, Insect ExpressionAn optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24. Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation pTriEx4 Add to Cart
65071RhoC FLARE.sc mCer, mVenus - wtRBD, mCerulean, mVenus, RhoCMammalian Expression, Bacterial Expression, Insect ExpressionA RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC pTriEx Add to Cart
65423RhoC FLARE.sc mCer, mVenus - DNRBD, mCerulean, mVenus, RhoCMammalian Expression, Bacterial Expression, Insect ExpressionA RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N pTriEx Add to Cart
65424RhoC FLARE.sc mCer, mVenus - CARBD, mCerulean, mVenus, RhoCMammalian Expression, Bacterial Expression, Insect ExpressionA RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L pTriEx Add to Cart
65456mVenus-PA-PKIPKI (Synthetic)Mammalian ExpressionManipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13. photoactivatable PKA inhibition pVenus-C1 PKIA PRKACN1 Pkia AI415001, PKIalpha Add to Cart
65457mVenus-PA-MKIMKI (Synthetic)Mammalian ExpressionManipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13. photoactivatable myosin light-chain kinase inihibition pVenus-C1 Add to Cart
75263PA-Cdc42Cell Division Cycle 42 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionA genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009 Sep 3. 461(7260):104-8. Light-activated Cdc42 pTriEx CDC42 CDC42Hs, G25K, TKS Add to Cart
81009pTriEx-NTOM20-LOV2LOV2 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Expression of LOV2 anchored to mitochondria pTriEx Add to Cart
81010pTriEx-NTOM20-mVenus-Zdk1Zdk1 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Expression of Zdk1 anchored to mitochondria pTriEx Add to Cart
81011pTriEx-NTOM20-mVenus-Zdk2Zdk2 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Expression of Zdk2 anchored to mitochondria pTriEx Add to Cart
81012pTriEx-NTOM20-mVenus-Zdk3Zdk3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Expression of Zdk1 anchored to mitochondria pTriEx Add to Cart
81031pTriEx-mCherry-LOV2 C450ALOV2 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Mammalian expression of the dark state of LOV2 pTriEx Add to Cart
81033pTriEx-mCherry-LOVSDLOVSD (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Mammalian expression of the super-dark state of LOV2 pTriEx Add to Cart
81035pTriEx-mCherry-LOV2 I427TLOV I427T (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. Mammalian expression of LOV I427T, a mutant with super short relaxation time pTriEx Add to Cart
81036pTriEx-mCherry-LOV2 V416LLOV V416L (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of LOV V416L, a mutant with super long relaxation time pTriEx Add to Cart
81040pTriEx-mCherry-LOV2 V416TLOV2 V416T (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of LOV V416T pTriEx Add to Cart
81041pTriEx-mCherry-LOV2LOV2 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression for wt LOV2 pTriEx Add to Cart
81042pTriEx-NLyn-Venus-Zdk1Zdk1 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of plasma-membrane anchored Zdk1 pTriEx Add to Cart
81044pTriEx-mCherry-LOV2 I427VLOV I427V (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of LOV2 I427V pTriEx Add to Cart
81045pTriEx-mCherry-LOV2 V416ILOV V416I (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of LOV V416I pTriEx Add to Cart
81046pTriEx-mVenus-Zdk1-RhoA Q63LZdk1 (Synthetic), RhoA Q63L (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged RhoA Q63L pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
81047pTriEx-mVenus-Zdk1Zdk1 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 pTriEx Add to Cart
81057pTriEx-mCherry-Zdk1Zdk1 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 pTriEx Add to Cart
81058pTriEx-mCherry-Zdk1-RhoA Q63LZdk1 (Synthetic), RhoA Q63L (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged RhoA Q63L pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
81059pTriEx-mCherry-Zdk1-Rac1 Q61LZdk1 (Synthetic), Rac1 Q61L (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged Rac1 Q61L pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
81060pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1Zdk1 (Synthetic), VAV2 DH/PH/C1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain pTriEx Add to Cart
81129pTriEx-mCherry-Zdk1-TIAM1 DH/PHZdk1 (Synthetic), TIAM1 DH/PH (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged Tiam1 Dh/PH pTriEx Add to Cart
81133pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1Zdk1 (Synthetic), VAV2 DH/PH/C1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain pTriEx Add to Cart
81137pTriEx-mVenus-Zdk1-Rac1 Q61LZdk1 (Synthetic), Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionLOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. mammalian expression of Zdk1 tagged Rac1 Q61L pTriEx RHOA ARH12, ARHA, RHO12, RHOH12 Add to Cart
83359pTriEX-Antenna-GDI Rac1Rac1 (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionFRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nat Chem Biol. 2016 Aug 8. doi: 10.1038/nchembio.2145. Expression of Antenna-Rac1 pTriEx RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
86974mCherry-PI(WT)-Vav2(DPZ)LOV2 (Synthetic), VAV2 DH/PH/C1 (Mus musculus)Mammalian ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2 pmCherry Vav2 2810040F13Rik, AI847175, Vav-2 Add to Cart
86977mCherry-PA(WT)-Vav2LOV2 (Synthetic), VAV2 DH/PH/ZF (Mus musculus)Mammalian ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(DM): Photo-activatable/light sensitive pmCherry Vav2 2810040F13Rik, AI847175, Vav-2 Add to Cart
86978pTriex-mVenus-PI-RhoA(Q63L)LOV2 (Synthetic), RhoA (Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(DM): Photo-activatable/light sensitive pTriEx Add to Cart
87357pBabe-PI(WT)-Src(YF)-mCherryLOV2 (Synthetic), Src isoform 2 (Mus musculus)Mammalian Expression, Bacterial ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation pBABE Src AW259666, pp60c-src Add to Cart
87358pBabe-PI(WT)-Src(KD)-mCherryLOV2 (Synthetic), Src (Mus musculus)Mammalian Expression, Bacterial ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation pBABE Src AW259666, pp60c-src Add to Cart
87550pBabe-PI(WT)-Rac1(WT)-mCherryLOV2 (Synthetic), Rac1 (Homo sapiens)Mammalian Expression, Bacterial ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(WT): Photo-inhibitable/blue light sensitive Rac1 pBABE RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
87553pBabe-PI(WT)-Rac1(Q61L)-mCherryLOV2 (Synthetic), Rac1 (Homo sapiens)Mammalian Expression, Bacterial ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1 pBABE RAC1 MIG5, Rac-1, TC-25, p21-Rac1 Add to Cart
87589PI(DM)-Rac1(WT)dc1.FLARELOV2 (Synthetic), PBD, Rac1Mammalian Expression, Bacterial Expression, Insect ExpressionEngineering extrinsic disorder to control protein activity in living cells. Science. 2016 Dec 16;354(6318):1441-1444. PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor pTriEx Add to Cart