Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET28a-EgC*
(Plasmid #20378)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20378 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5367
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Eglin C wt*
  • Species
    Leech
  • Insert Size (bp)
    216
  • Mutation
    F10W
  • GenBank ID
    X00802

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr Marshall Edgell (UNC Microbiology and Immunology)
  • Terms and Licenses

Depositor Comments

PDZ2 was amplified out of hPTP1e cDNA and ligated into pET21a between sites NdeI (5') and BamHI (3')

IMPORTANT: Please note that the His tag in this Pet28 vector is NOT in frame with the Eglin C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-EgC* was a gift from Andrew Lee (Addgene plasmid # 20378 ; http://n2t.net/addgene:20378 ; RRID:Addgene_20378)
  • For your References section:

    Increased rigidity of eglin c at acidic pH: evidence from NMR spin relaxation and MD simulations. Hu H, Clarkson MW, Hermans J, Lee AL. Biochemistry. 2003 Dec 2. 42(47):13856-68. 10.1021/bi035015z PubMed 14636053