Depositor Sequences: None.
Addgene Sequences: Partial (1)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11452||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3188
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameintracellular protease
Insert Size (bp)498
GenBank IDBAA30818 PH1704 NP_143548
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pHMMa.PH1704 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHMMa.PH1704 was a gift from Sung-Hou Kim (Addgene plasmid # 11452)
For your References section:Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH. Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14079-84. 10.1073/pnas.260503597 PubMed 11114201
Generated by Addgene from plasmid data.