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(Plasmid #49048)


Item Catalog # Description Quantity Price (USD)
Plasmid 49048 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6392
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    Enteropeptidase, peptidase domain
  • Alt name
  • Species
    B. taurus (bovine)
  • Mutation
    P92R, E185D, C122S
  • GenBank ID
  • Entrez Gene
    TMPRSS15 (a.k.a. PRSS7)
  • Promoter T7
  • Tag / Fusion Protein
    • 5xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Haidong Tan (Dalian Institute of Chemical Physics, PR China), see Tan et al., Protein Expr. Purif. 56 (2007), 40–47
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b-EK_C122S_His5 was a gift from Hans Brandstetter (Addgene plasmid # 49048 ; ; RRID:Addgene_49048)
  • For your References section:

    Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox. Skala W, Goettig P, Brandstetter H. J Biotechnol. 2013 Dec;168(4):421-5. doi: 10.1016/j.jbiotec.2013.10.022. Epub 2013 Oct 30. 10.1016/j.jbiotec.2013.10.022 PubMed 24184090