PurposeExpresses untagged human rhinovirus 3C protease in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78571||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6358
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsExpress in BL21(DE3) cells
Copy numberLow Copy
Gene/Insert namehuman rhinovirus 3C protease
SpeciesH. sapiens (human)
- Promoter T7
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSRK2706 was a gift from David Waugh (Addgene plasmid # 78571 ; http://n2t.net/addgene:78571 ; RRID:Addgene_78571)
For your References section:A dual protease approach for expression and affinity purification of recombinant proteins. Raran-Kurussi S, Waugh DS. Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006. 10.1016/j.ab.2016.04.006 PubMed 27105777