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pFR_CrPV_xb
(Plasmid #11509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11509 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 11509-D50 50 μg of DNA in Tris buffer $495
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DNA 11509-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see comments (unknown if destroyed)
  • 3′ cloning site see comments (unknown if destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CrPV IRES sequence was cloned by splint ligation of two synthetic 100mers, and cloned into the 5' UTR of pRL-TKx6; the firefly luciferase ORF was then inserted into the resulting construct. Six short RNA binding sites for CXCR4 are also present.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFR_CrPV_xb was a gift from Phil Sharp (Addgene plasmid # 11509 ; http://n2t.net/addgene:11509 ; RRID:Addgene_11509)

  • For your References section:

    Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. Mol Cell. 2006 Feb 17. 21(4):533-42. 10.1016/j.molcel.2006.01.031 PubMed 16483934
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