Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #11510)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11510 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4045
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see comments (unknown if destroyed)
  • 3′ cloning site see comments (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid constructed by PCR cloning HCV IRES into the NheI site of pRL-TKx6 and inserting the Firefly luciferase ORF from pGL3 into the resulting vectorl.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFR_HCV_xb was a gift from Phil Sharp (Addgene plasmid # 11510 ; ; RRID:Addgene_11510)
  • For your References section:

    Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. Mol Cell. 2006 Feb 17. 21(4):533-42. 10.1016/j.molcel.2006.01.031 PubMed 16483934