pFR_CrPV_xb
(Plasmid #11509)

• Depositing Lab: Phil Sharp
• Publication: Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Backbone

• Vector backbone: pRL-TK
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4045
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: see comments (unknown if destroyed)
• 3′ cloning site: see comments (unknown if destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CrPV IRES sequence was cloned by splint ligation of two synthetic 100mers, and cloned into the 5' UTR of pRL-TKx6; the firefly luciferase ORF was then inserted into the resulting construct. Six short RNA binding sites for CXCR4 are also present.