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Addgene

pAAV2-TBG-FLAG-mRIPK1
(Plasmid #115341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV2
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RIPK1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ripk1 (a.k.a. D330015H01Rik, RIP, RIP-1, Rinp, Rip1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV2-TBG-FLAG-mRIPK1 was a gift from Junying Yuan (Addgene plasmid # 115341 ; http://n2t.net/addgene:115341 ; RRID:Addgene_115341)

  • For your References section:

    Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis. Geng J, Ito Y, Shi L, Amin P, Chu J, Ouchida AT, Mookhtiar AK, Zhao H, Xu D, Shan B, Najafov A, Gao G, Akira S, Yuan J. Nat Commun. 2017 Aug 25;8(1):359. doi: 10.1038/s41467-017-00406-w. 10.1038/s41467-017-00406-w [pii] PubMed 28842570
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