|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104963||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backbonepUC19 with additional elements
- Total vector size (bp) 7468
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)4090
- Promoter p5
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV2/2 was a gift from Melina Fan (Addgene plasmid # 104963 ; http://n2t.net/addgene:104963 ; RRID:Addgene_104963)