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pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
(Plasmid #115432)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115432 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 5527
  • Modifications to backbone
    Yeast ARS/CEN
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RPL28
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1023
  • Mutation
    exon 2 deleted, with MfeI / NheI cloning site
  • GenBank ID
    NM_001180968
  • Promoter endogenous

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HIS5
  • Species
    S. pombe (fission yeast); Candida albicans
  • Insert Size (bp)
    1425
  • Promoter Ashbya gospii TEF1

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619) was a gift from Nicholas Ingolia (Addgene plasmid # 115432 ; http://n2t.net/addgene:115432 ; RRID:Addgene_115432)
  • For your References section:

    An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries. McGeachy AM, Meacham ZA, Ingolia NT. ACS Synth Biol. 2019 Apr 19;8(4):844-856. doi: 10.1021/acssynbio.8b00529. Epub 2019 Apr 2. 10.1021/acssynbio.8b00529 PubMed 30908907