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An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries.
McGeachy AM, Meacham ZA, Ingolia NT
ACS Synth Biol. 2019 Apr 19;8(4):844-856. doi: 10.1021/acssynbio.8b00529. Epub 2019 Apr 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
115429pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)Selection for in-frame fusion expression constructs
115431pRPL28 CaURA3 (pNTI592)URA3-marked RPS28
115432pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)Vector for expressing RPL28 with mutagenized exon2
115433pRPL28 SpHIS5 (pNTI663)Expresses wild-type RPL28 from HIS3 yeast plasmid
115434pRPL28(Q38P) SpHIS5 (pNTI664)Expresses Q38P RPL28 from HIS3 yeast plasmid
115435pRPL28(H39L) SpHIS5 (pNTI665)Expresses H39L RPL28 from HIS3 yeast plasmid
115436pRPL28(K55M) SpHIS5 (pNTI666)Expresses K55M RPL28 from HIS3 yeast plasmid

Antibodies from Article