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Depositing Lab
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Depositing OrganizationBoston Children's Hospital
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 11739-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 11739-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBSII KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
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Vector typeCre/Lox ; /STOP
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Selectable markersNeomycin (select with G418) ; Diptheriatoxin A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTOP-eGFP-ROSA26TV
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SpeciesDNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
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Insert Size (bp)11500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer T7/M13
- 3′ sequencing primer T7/M13
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byBased on pRosa26-1 from Soriano P, Fred Hutchinson, Cancer Research Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Targeting vector for cDNA knock-in into the Rosa26 locus.
See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912
DNA (Catalog # 11739-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 11739-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STOP-eGFP-ROSA26TV was a gift from Klaus Rajewsky (Addgene plasmid # 11739 ; http://n2t.net/addgene:11739 ; RRID:Addgene_11739) -
For your References section:
Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, Schmidt-Supprian M. Immunity. 2006 Jun . 24(6):729-39. 10.1016/j.immuni.2006.04.005 PubMed 16782029
Map uploaded by the depositor.
