|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15036||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepRosa26 PA
- Backbone size w/o insert (bp) 9896
Vector typefor making transgenic constructs
Growth in Bacteria
Gene/Insert namepRosa26 modified targeting vector
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Modification of plasmid pRosa26PA described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. See BMC Dev Biol article for more information.
Clone into PacI & AscI sites. The modified plasmid conatins additional sites for linearization. See Author's Map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRosa26PAm1 (DM#272) was a gift from Douglas Melton (Addgene plasmid # 15036 ; http://n2t.net/addgene:15036 ; RRID:Addgene_15036)
For your References section:Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333