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pRosa26PAm1 (DM#272)
(Plasmid #15036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15036 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 15036-D50 50 μg of DNA in Tris buffer $495
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DNA 15036-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRosa26 PA
  • Backbone size w/o insert (bp) 9896
  • Vector type
    for making transgenic constructs

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pRosa26 modified targeting vector

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pRosa26PA from Frank Costantini.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modification of plasmid pRosa26PA described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. See BMC Dev Biol article for more information.

Clone into PacI & AscI sites. The modified plasmid conatins additional sites for linearization. See Author's Map for more information.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRosa26PAm1 (DM#272) was a gift from Douglas Melton (Addgene plasmid # 15036 ; http://n2t.net/addgene:15036 ; RRID:Addgene_15036)

  • For your References section:

    Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333
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